Document Detail

Age-dependent recruitment of neutrophils by fetal endothelial cells: implications in scarless wound healing.
MedLine Citation:
PMID:  17208559     Owner:  NLM     Status:  MEDLINE    
PURPOSE: Fetal dermal wounds heal with minimal inflammation and absent fibrosis. Later in gestation, a transition to adult-like healing with marked inflammation and scarring is observed. Interaction with endothelial cells (ECs) is imperative for leukocyte transmigration, a critical step in the inflammatory cascade. This study was embarked upon to determine if gestational age-dependent differences in EC function modulate changes in inflammatory response and correlate with the healing phenotype. METHODS: Fetal porcine ECs were harvested at days 65 (mid gestation), 85 (late gestation), and 100 (near-term) (term = 115 days). Confluent monolayers were activated with IL-1beta at 10 and 100 ng/mL and exposed to adult neutrophils under static (n = 4 per group) and continuous flow (n = 6 per group) conditions. Neutrophil-endothelial interaction was quantified and compared using analysis of variance. RESULTS: Under static conditions, the lower cytokine dose elicited maximal neutrophil recruitment in later-gestation ECs, while midgestation ECs required higher stimulation. Midgestation ECs recruited significantly less neutrophils than later gestation ECs at both cytokine concentrations under flow conditions. CONCLUSION: There is a gestational age-dependent variation in neutrophil recruitment by fetal ECs. With minimal stimulation, later-gestation ECs actively recruit neutrophils, whereas midgestation ECs do not. These findings correlate with the transition period to adult-like healing, supporting the potential role of fetal ECs in scarless healing.
Bindi Naik-Mathuria; A Nicolas Gay; Xi Zhu; Ling Yu; Darrell L Cass; Oluyinka O Olutoye
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of pediatric surgery     Volume:  42     ISSN:  1531-5037     ISO Abbreviation:  J. Pediatr. Surg.     Publication Date:  2007 Jan 
Date Detail:
Created Date:  2007-01-08     Completed Date:  2007-02-05     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  0052631     Medline TA:  J Pediatr Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  166-71     Citation Subset:  IM    
Division of Pediatric Surgery, Michael E. DeBakey Department of Surgery, Baylor College of Medicine, Houston, TX 77030-2399, USA.
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MeSH Terms
Age Factors
Cells, Cultured
Cicatrix / immunology*
Endothelial Cells / immunology*
Fetus / immunology
Gestational Age
Neutrophil Infiltration / immunology*
Wound Healing / immunology*
Grant Support

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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