| Age dependent incorporation of 14C-DHA into rat brain and body tissues after dosing various 14C-DHA-esters. | |
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MedLine Citation:
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PMID: 20580213 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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INTRODUCTION: The omega-3 fatty acid docosahexaenoic acid (DHA) accounts for 10% of fatty acids in human brain and is critical for neuronal function and brain development. Mechanisms of transport, accumulation and conservation of DHA in the brain are unclear. The objective of the study was to quantify the age dependent DHA incorporation into the brain of 2-, 4- or 10-week-old rats after a bolus dose of different DHA-esters. METHODS: Rats were gavaged with (14)C-DHA-TAG, (14)C-DHA-PL or (14)C-DHA-TAG+PL at 2 mg DHA/kg BW. After 24h the distribution of radioactivity in body and brain regions was determined using quantitative whole body autoradiography (QWBA). Radiolabeled compounds were extracted from the brains to determine the identity of the radiolabeled compounds. RESULTS: Accumulation of orally ingested (14)C-DHA in rat brain was less than 1% of the dose and decreased with age. Ester specific differences were seen only in 10-week-old rats, where oral (14)C-DHA-PL delivered a 2-fold higher accretion of radioactivity in the brain. CONCLUSIONS: Less than 1% of a dietary achievable DHA dose reached the rat brain within 24h. Optimal efficacy of DHA-PL may occur in older age groups. |
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Authors:
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B A Graf; G S M J E Duchateau; A B Patterson; E S Mitchell; P van Bruggen; J H Koek; S Melville; H J Verkade |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-26 |
Journal Detail:
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Title: Prostaglandins, leukotrienes, and essential fatty acids Volume: 83 ISSN: 1532-2823 ISO Abbreviation: Prostaglandins Leukot. Essent. Fatty Acids Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-08-06 Completed Date: 2010-09-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8802730 Medline TA: Prostaglandins Leukot Essent Fatty Acids Country: Scotland |
Other Details:
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Languages: eng Pagination: 89-96 Citation Subset: IM |
Copyright Information:
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(c) 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Unilever R&D Vlaardingen, PO Box 114, 3130AC Vlaardingen, The Netherlands. brigitte.graf@unilever.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aging
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metabolism* Animals Biological Transport / drug effects, physiology Brain / metabolism* Brain Chemistry / drug effects*, physiology Carbon Isotopes / metabolism, pharmacology Docosahexaenoic Acids / metabolism*, pharmacology* Dose-Response Relationship, Drug Humans Male Rats Rats, Wistar Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Carbon Isotopes; 25167-62-8/Docosahexaenoic Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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