Document Detail


Age-dependent fate and lineage restriction of single NG2 cells.
MedLine Citation:
PMID:  21266410     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
NG2-expressing glia (NG2 cells, polydendrocytes) appear in the embryonic brain, expand perinatally, and persist widely throughout the gray and white matter of the mature central nervous system. We have previously reported that NG2 cells generate oligodendrocytes in both gray and white matter and a subset of protoplasmic astrocytes in the gray matter of the ventral forebrain and spinal cord. To investigate the temporal changes in NG2 cell fate, we generated NG2creER™BAC transgenic mice, in which tamoxifen-inducible Cre is expressed in NG2 cells. Cre induction at embryonic day 16.5, postnatal day (P) 2, P30 and P60 in mice that were double transgenic for NG2creER™BAC and the Cre reporter revealed that NG2 cells in the postnatal brain generate only NG2 cells or oligodendrocytes, whereas NG2 cells in the embryonic brain generate protoplasmic astrocytes in the gray matter of the ventral forebrain in addition to oligodendrocytes and NG2 cells. Analysis of cell clusters from single NG2 cells revealed that more than 80% of the NG2 cells in the P2 brain give rise to clusters consisting exclusively of oligodendrocytes, whereas the majority of the NG2 cells in the P60 brain generate clusters that contain only NG2 cells or a mixture of oligodendrocytes and NG2 cells. Furthermore, live cell imaging of single NG2 cells from early postnatal brain slices revealed that NG2 cells initially divide symmetrically to produce two daughter NG2 cells and that differentiation into oligodendrocytes occurred after 2-3 days.
Authors:
Xiaoqin Zhu; Robert A Hill; Dirk Dietrich; Mila Komitova; Ryusuke Suzuki; Akiko Nishiyama
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  138     ISSN:  1477-9129     ISO Abbreviation:  Development     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-26     Completed Date:  2011-03-02     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  745-53     Citation Subset:  IM    
Affiliation:
Department of Physiology and Neurobiology, University of Connecticut, Storrs, CT 06269-3156, USA.
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MeSH Terms
Descriptor/Qualifier:
Aging*
Animals
Antigens / genetics,  metabolism*
Cell Differentiation
Cell Lineage*
Gene Expression Regulation, Developmental
Mice
Mice, Transgenic
Oligodendroglia / cytology,  metabolism
Promoter Regions, Genetic
Proteoglycans / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Antigens; 0/Proteoglycans; 0/chondroitin sulfate proteoglycan 4
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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