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Age-dependent effect of ouabain on renal Na(+),K(+)-ATPase.
MedLine Citation:
PMID:  21338612     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Aims This study examines the effect of chronic ouabain-treatment on renal Na(+) handling in 12-week and 52-week old rats. Main methods Wistar Kyoto rats aged 5-weeks or 45-weeks were treated with ouabain or vehicle during 7weeks. Blood pressure was measured in conscious animals throughout the study. After 7weeks of treatment urinary electrolyte concentration, Na(+),K(+)-ATPase activity and α(1)-subunit expression were determined in 12-week and 52-week old rats. Key findings In 12-week and 52-week old rats ouabain produced a significant increase in systolic blood pressure. Although no differences were observed in Na(+) excretion in these animals, 12-week old ouabain-treated rats had lower Na(+),K(+)-ATPase activity in proximal tubules. However, 12-week old ouabain-treated rats had decreased fractional excretion of Na(+). In proximal tubules of 52-week old rats Na(+),K(+)-ATPase activity did not differ between vehicle and ouabain-treated groups. Significance Our results show that in Wistar Kyoto rats renal response to ouabain treatment may be age-dependent and that the hypertensive effect of ouabain is independent of the effect on renal Na(+),K(+)-ATPase.
Authors:
E Silva; M P Serrão; P Soares-da-Silva
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-2-18
Journal Detail:
Title:  Life sciences     Volume:  -     ISSN:  1879-0631     ISO Abbreviation:  -     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-2-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2010. Published by Elsevier Inc.
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