Document Detail


Age-dependent changes in the specificity of tRNA methyltransferases in the cerebellum of the icteric and nonicteric Gunn rat.
MedLine Citation:
PMID:  492445     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The activity of tRNA methyltransferases present in the cerebellum of 6- and 21-day-old nonicteric and icteric Gunn rats was compared using purified E. coli tRNAs as substrates. At 6 days the tRNA methyltransferases of the icteric animals were significantly more effective in methylating tRNAGlu2 and tRNAPhe than were those of their nonicteric counterparts. This relationship reversed itself at 21 days. The action of the tRNA methyltransferases from the 6-day-old icteric animals led to higher proportions of 1-methyladenine in tRNAGlu2 and tRNAPhe than were obtained using the corresponding enzymes of the nonicteric animals. The proportion of N2-methylguanine was also higher, yet only in tRNAfMet and not in tRNAPhe. The study reveals much more extensive fluctuations in the activity and in the substrate recognition specificity among the cerebellar tRNA methyltransferases of the icteric than among those of the nonicteric controls during the crucial 6--21 day period of cerebellar development.
Authors:
J Dainat; F de Balbian Verster; R Zand; O Z Sellinger
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Neurochemical research     Volume:  4     ISSN:  0364-3190     ISO Abbreviation:  Neurochem. Res.     Publication Date:  1979 Oct 
Date Detail:
Created Date:  1979-12-27     Completed Date:  1979-12-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  557-65     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aging
Animals
Cerebellum / enzymology*
Hyperbilirubinemia / metabolism*
Jaundice / metabolism*
Organ Size
Rats
tRNA Methyltransferases / metabolism*
Chemical
Reg. No./Substance:
EC 2.1.1.-/tRNA Methyltransferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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