| Age-dependent changes in the heat-stable crystallin, beta Bp, of the human lens. | |
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MedLine Citation:
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PMID: 3720346 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The present study examined the effects of aging and cataractogenesis on the biochemical properties of the uniquely heat-stable lens crystallin, beta basic principle polypeptide (beta Bp). Using the techniques of SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western-blot immunoassay, we analyzed cortical and nuclear lens sections from normal lenses of individuals aged 0 to 91 years for beta Bp content in the soluble fraction, and retention of heat stability with aging. In addition, we compared the characteristics of beta Bp in cataractous lenses with those of normal lenses of approximately the same age. While beta Bp is synthesized in new cortical cells throughout life, the beta Bp of the nucleus, which had been laid down early in life, decreased significantly in both absolute concentration and in its proportion of the total soluble protein fraction during the normal aging process. In addition, posttranslational changes in the nuclear soluble beta Bp result in a gradual loss of approximately 3000 d in the apparent mass of the beta Bp molecule; i.e., as a result of the aging process, the single heat-stable band of an apparent mass of 26 kd on SDS-PAGE of the young lens nucleus becomes two heat-stable bands, one at 26 kd and one at 23 kd. Normal lenses up to 91 years of age always retain some of the 26 kd subunit, whereas lenses with severe nuclear cataracts had only the 23 kd subunit in the soluble fraction. |
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Authors:
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M McFall-Ngai; J Horwitz; L L Ding; L Lacey |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Current eye research Volume: 5 ISSN: 0271-3683 ISO Abbreviation: Curr. Eye Res. Publication Date: 1986 May |
Date Detail:
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Created Date: 1986-08-18 Completed Date: 1986-08-18 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8104312 Medline TA: Curr Eye Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 387-94 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Aged Aging* Cataract / metabolism Child Child, Preschool Crystallins / metabolism Drug Stability Hot Temperature* Humans Infant Infant, Newborn Lens Cortex, Crystalline / metabolism Lens, Crystalline / metabolism* Middle Aged Tissue Distribution |
| Grant Support | |
ID/Acronym/Agency:
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EY 0331/EY/NEI NIH HHS; EY 3897/EY/NEI NIH HHS; EY 5905/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Crystallins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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