Document Detail


Age dependent aneuploidy and telomere length of the human vascular endothelium.
MedLine Citation:
PMID:  11730807     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
RATIONALE: Aneuploidy and telomere length are two major parameters that have been associated with cellular senescence in vitro. In order to explore the role of aneuploidy and telomere length in aging of the human vasculature, we studied these two parameters in direct preparations of endothelial cells of the human abdominal aorta. METHODS: Using fluorescent in situ hybridization on 'touch prep' slides, we evaluated aneuploidy of two autosomes (chromosomes 6 and 16) and sex chromosomes in non cultured endothelial cells of the abdominal aorta as a function of the donor's age. RESULTS: We found that the frequency of aneuploidy of vascular endothelial cells significantly increased with age. This was expressed by age-dependent tetrasomy (r(s)=0.56, P=0.006 for chromosome 6; and r(s)=0.54, P=0.008 for chromosome 16), and age dependent loss of the Y chromosome (r(s)=0.85, P=0.0003). In addition, we found that telomere length was inversely correlated with age (r=-0.38, P=0.008). DATA INTERPRETATION: These findings suggest that indicators of cellular senescence, earlier observed in vitro, are also expressed in the human vascular endothelium in vivo. Aneuploidy and telomere attrition might thus play a role in the aging of the human vasculature.
Authors:
H Aviv; M Y Khan; J Skurnick; K Okuda; M Kimura; J Gardner; L Priolo; A Aviv
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Atherosclerosis     Volume:  159     ISSN:  0021-9150     ISO Abbreviation:  Atherosclerosis     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2001-12-03     Completed Date:  2002-02-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0242543     Medline TA:  Atherosclerosis     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  281-7     Citation Subset:  IM    
Affiliation:
The Center of Human and Molecular Genetics, UMDNJ-New Jersey Medical School, 185 South Orange Avenue, Newark, NJ 07103, USA.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Aged, 80 and over
Aging / physiology*
Aneuploidy*
Aorta, Abdominal
Cells, Cultured
Child
Child, Preschool
DNA / analysis
Endothelium, Vascular / cytology,  physiology*
Female
Gene Expression
Humans
In Situ Hybridization, Fluorescence
Infant
Male
Middle Aged
Sensitivity and Specificity
Telomere / genetics*,  physiology
Grant Support
ID/Acronym/Agency:
HL-47906/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
9007-49-2/DNA

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