Document Detail

Age- and cell cycle-dependent changes in EPC-1/PEDF promoter activity in human diploid fibroblast-like (HDF) cells.
MedLine Citation:
PMID:  16896539     Owner:  NLM     Status:  MEDLINE    
The changes in gene expression during senescence are very interesting. Early population doubling cDNA-1 (EPC-1, also known as pigment epithelial derived factor, PEDF) is one of the genes whose expression decreases dramatically during cellular aging. We examined whether or not EPC-1/PEDF promoter activity was affected by the cellular ageing using human diploid lung fibroblast cells in culture. Here we showed the promoter/enhancer region of EPC-1/PEDF existed at more than 1760 bp upstream from the transcriptional initiation site of the gene, and was regulated by both aging and cell cycle. These findings suggest that the expression of the EPC-1/PEDF gene is, at least in part, regulated transcriptionally in the cells. The analysis of the promoter region of the EPC-1/PEDF gene in this paper suggests the age- and cell cycle-dependent expression of specific transcriptional factor(s).
Toshihiko Kojima; Ken-ichi Nakahama; Kiyotaka Yamamoto; Hiroshi Uematsu; Ikuo Morita
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-07-31
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  293     ISSN:  0300-8177     ISO Abbreviation:  Mol. Cell. Biochem.     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-11-30     Completed Date:  2007-03-16     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  63-9     Citation Subset:  IM    
Department of Cellular Physiological Chemistry, Graduated School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan.
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MeSH Terms
Cell Aging / genetics*
Cell Cycle / genetics*,  physiology
Cells, Cultured
Eye Proteins / genetics*,  metabolism
Fibroblasts / metabolism*
Nerve Growth Factors / genetics*,  metabolism
Promoter Regions, Genetic*
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Serpins / genetics*,  metabolism
Transcription Initiation Site
Transcription, Genetic
Reg. No./Substance:
0/Eye Proteins; 0/Nerve Growth Factors; 0/RNA, Messenger; 0/Serpins; 0/pigment epithelium-derived factor

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