Document Detail

Age-associated alterations in the recruitment of signal-transduction proteins to lipid rafts in human T lymphocytes.
MedLine Citation:
PMID:  14657209     Owner:  NLM     Status:  MEDLINE    
Aging is associated with a decline in T cell activation and proliferation, but the underlying mechanisms are not fully understood. Recent findings suggest that lipid rafts act as a platform in the initiation of T cell activation by selectively recruiting signaling proteins associated with formation of the initial complex of signal transduction. We tested the hypothesis that lipid raft properties are altered in T lymphocytes from elderly, healthy individuals in comparison with young subjects. Results showed that the cholesterol content of lipid rafts derived from these cells was consistently higher in the case of elderly donors and that membrane fluidity was decreased. In addition, lipid rafts coalescence to the site of T cell receptor engagement was impaired in T cells from elderly donors. The recruitment of p56(lck), linker of activated T cells, and their tyrosine-phosphorylated forms to lipid rafts was decreased in activated T cells from aged individuals. CD45 was not recruited to the lipid raft fractions in either group of subjects. Our data suggest that some properties of lipid rafts are altered in aging, and this finding may be part of the causes for the decline in T cell functions that are observed in elderly individuals.
Anis Larbi; Nadine Douziech; Gilles Dupuis; Abdelouahed Khalil; Hugues Pelletier; Karl-Philippe Guerard; Tamàs Fülöp
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-12-04
Journal Detail:
Title:  Journal of leukocyte biology     Volume:  75     ISSN:  0741-5400     ISO Abbreviation:  J. Leukoc. Biol.     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-02-05     Completed Date:  2004-03-26     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8405628     Medline TA:  J Leukoc Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  373-81     Citation Subset:  IM    
Research Center on Aging, Geriatric Institute, Clinical Research Center, University of Sherbrooke, 1036 Belvedere St. South, Sherbrooke, QC, Canada J1H 4C4.
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MeSH Terms
Aging / immunology*
Antigens, CD45 / metabolism
Cholesterol / analysis
Lymphocyte Activation
Lymphocyte Specific Protein Tyrosine Kinase p56(lck) / metabolism
Membrane Fluidity
Membrane Microdomains / chemistry,  metabolism*
Membrane Proteins / metabolism*
Protein Transport
Receptors, Antigen, T-Cell / metabolism
Signal Transduction*
T-Lymphocytes / metabolism*
Reg. No./Substance:
0/Membrane Proteins; 0/Receptors, Antigen, T-Cell; 57-88-5/Cholesterol; EC Specific Protein Tyrosine Kinase p56(lck); EC, CD45

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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