Document Detail


Age-related pseudocapillarization of the liver sinusoidal endothelium impairs the hepatic clearance of acetaminophen in rats.
MedLine Citation:
PMID:  21300741     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the effect of age-related pseudocapillarization of the liver sinusoidal endothelium on the hepatic disposition of acetaminophen. The multiple indicator dilution technique assessed the hepatic disposition of tracer (14)C-acetaminophen and reference markers in isolated perfused livers of young (n = 11) and old (n = 12) rats. Electron microscopy confirmed defenestration of the sinusoidal endothelium in old rats compared with young rats. Acetaminophen recovery following a single pass through the liver was significantly increased in old rats (0.64 ± 0.04, old; 0.59 ± 0.05, young; p < .05). In old age, there was significant reduction of the intercompartmental rate constant k(1) (0.34 ± 0.10 s(-1), old; 0.61 ± 0.38 s(-1), young; p < .05) and the permeability-surface area product for the transfer of acetaminophen across the sinusoidal endothelium (0.034 ± 0.006 mL/s/g, old; 0.048 ± 0.014 mL/s/g, young; p < .005). There was no difference in k(3), the measure of sequestration of acetaminophen that reflects enzyme activity. Age-related pseudocapillarization of the liver sinusoid resulted in increased acetaminophen recovery and decreased transfer of acetaminophen into the liver.
Authors:
Sarah J Mitchell; Aniko Huizer-Pajkos; Victoria C Cogger; Andrew J McLachlan; David G Le Couteur; Brett Jones; Rafael de Cabo; Sarah N Hilmer
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't     Date:  2011-02-07
Journal Detail:
Title:  The journals of gerontology. Series A, Biological sciences and medical sciences     Volume:  66     ISSN:  1758-535X     ISO Abbreviation:  J. Gerontol. A Biol. Sci. Med. Sci.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-03-14     Completed Date:  2011-06-02     Revised Date:  2012-04-02    
Medline Journal Info:
Nlm Unique ID:  9502837     Medline TA:  J Gerontol A Biol Sci Med Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  400-8     Citation Subset:  AIM; IM    
Affiliation:
Laboratory of Ageing and Pharmacology, Kolling Institute of Medical Research, Royal North Shore Hospital, Sydney, Australia. sarah.mitchell@sydney.edu.au
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MeSH Terms
Descriptor/Qualifier:
Acetaminophen / pharmacokinetics*
Aging
Analgesics, Non-Narcotic / pharmacokinetics*
Animals
Antipyretics / pharmacokinetics*
Endothelium / metabolism
Indicator Dilution Techniques
Liver / blood supply,  metabolism*,  ultrastructure
Male
Microscopy, Electron, Scanning
Rats
Rats, Inbred F344
Chemical
Reg. No./Substance:
0/Analgesics, Non-Narcotic; 0/Antipyretics; 103-90-2/Acetaminophen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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