Document Detail


Agarose-gelatin conjugate for adherent cell-enclosing capsules.
MedLine Citation:
PMID:  17333466     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Spherical capsules were prepared by extruding aqueous agarose-gelation conjugate solution into co-flowing liquid paraffin at 38 degrees C and cooling the resultant emulsion. Capsule diameter was controlled between 40 and 250 mum by changing the velocity of the liquid paraffin. Adherent Crandall-Reese feline kidney cells enclosed in conjugate capsules of 141 +/- 23 mum diam. had a higher degree of proliferation than those in unmodified agarose capsules. Mitochondrial activity, detected for cell-enclosing conjugate capsules normalized against unit volume of gel, was about double that of unmodified agarose capsules over 28 days. These results demonstrated the feasibility of agarose-gelatin conjugate as a material of cell-enclosing capsules.
Authors:
Shinji Sakai; Ichiro Hashimoto; Koei Kawakami
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-02-28
Journal Detail:
Title:  Biotechnology letters     Volume:  29     ISSN:  0141-5492     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-04-06     Completed Date:  2007-08-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  731-5     Citation Subset:  IM    
Affiliation:
Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan. sakai@chem-eng.kyushu-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Capsules / metabolism*
Cats
Cell Adhesion
Cell Line
Gelatin / metabolism*
Mineral Oil
Mitochondria / metabolism
Particle Size
Sepharose / metabolism*
Chemical
Reg. No./Substance:
0/Capsules; 8020-83-5/Mineral Oil; 9000-70-8/Gelatin; 9012-36-6/Sepharose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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