Document Detail

Agar media that indicate acid production from sorbitol by oral microorganisms.
MedLine Citation:
PMID:  2933424     Owner:  NLM     Status:  MEDLINE    
Two varieties of agar medium (Trypticase [BBL Microbiology Systems]-serum-sorbitol-bromcresol purple agar [TSSB] and Trypticase-blood-sorbitol-CaCO3 agar [TBSCa]) indicating microbial acid production from sorbitol were tested. The media were devised for use in studies on the prevalence of sorbitol-fermenting human oral microorganisms incubated in an anaerobic or microaerophilic atmosphere containing 5 to 6% CO2. TSSB contains bromcresol purple as the pH indicator and NaHCO3 as the main buffering salt. TBSCa contains CaCO3 as both the buffering salt and the indicator of acid production. The growth yield of pure cultures of oral microorganisms on TBSCa was shown to equal that on blood agar incubated under similar conditions. TSSB inhibited the growth of several bacteria to various extents. The recovery of sorbitol-fermenting microorganisms from oral specimens was the greatest when the specimens were assayed with TBSCa. The poorer results obtained with TSSB were mainly due to the decoloration of the pH indicator in this medium and the presence of greater numbers of sorbitol false-positive colonies.
S Kalfas; S Edwardsson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  22     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  1985 Dec 
Date Detail:
Created Date:  1986-01-17     Completed Date:  1986-01-17     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  959-61     Citation Subset:  IM    
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MeSH Terms
Acids / metabolism
Bacteria / isolation & purification,  metabolism*
Bacteriological Techniques
Culture Media
Mouth / microbiology*
Protein Hydrolysates
Sorbitol / metabolism*
Reg. No./Substance:
0/Acids; 0/Caseins; 0/Culture Media; 0/Protein Hydrolysates; 0/trypticase-soy broth; 50-70-4/Sorbitol; 9002-18-0/Agar

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