Document Detail


Ag-staining of nucleolus organizer regions of chromosomes after A-,C-, G-, or R-banding procedures.
MedLine Citation:
PMID:  69519     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Metaphase chromosome preparations were made from leukocyte cultures of normal individuals. The cells were fixed in methanol:acetic acid (3:1 v/v), then dropped on cold, wet slides which were air-dried before storage at 4 degrees C. The slides were stained to identify the chromosomes by one of the following procedures: (1) Quinacrine. Slides were stained for 10 min in quinacrine mustard solution, rinsed in running tap water for 2 min, and mounted in Tris-maleat buffer, pH 5.6.
Authors:
R Tantravahi; D A Miller; O J Miller
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cytogenetics and cell genetics     Volume:  18     ISSN:  0301-0171     ISO Abbreviation:  Cytogenet. Cell Genet.     Publication Date:  1977  
Date Detail:
Created Date:  1977-09-29     Completed Date:  1977-09-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0367735     Medline TA:  Cytogenet Cell Genet     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  364-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Nucleolus / ultrastructure*
Chromosomes / ultrastructure*
Humans
Mitosis
Silver / diagnostic use*
Staining and Labeling
Chemical
Reg. No./Substance:
7440-22-4/Silver

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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