| Ag+ alters cell growth, neurite extension, cardiomyocyte beating, and fertilized egg constriction. | |
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MedLine Citation:
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PMID: 10608607 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The Russian Space Agency uses electrochemically generated silver ions (Ag+) to purify drinking water for their space station, Mir, and their portion of the International Space Station. U.S. EPA guidelines allow 10.6 micromol x L(-1) Ag+ in human drinking water for up to 10 d. Studies correlate Ag+ exposure with tissue dysfunction in humans, rats, and mice, and with altered ion transport, skeletal muscle contraction, and embryonic cell constriction in other animal cells. Ag+ effects on cell shape change-related functions have not been assessed. METHODS: Immortalized embryonic human intestinal epithelial cells, freshly explanted embryonic avian nerve cells and cardiomyocytes, and marine fertilized eggs were grown in vitro in medium containing AgNO3. RESULTS: Intestinal cells detach from the substratum and viable cell number decreases by 5-6 d at 5 micromol x L(-1) AgNO3, and faster at higher concentrations. Microtubules appear unaltered in adherent cells. Detached cells are nonviable. Neurite outgrowth and glial cell migration from dorsal root ganglia are inhibited by 3 d at 15 micromol x L(-1) AgNO3 or greater. Contractions stop temporarily in most cardiomyocytes by 5 min at 5 micromol x L(-1) AgNO3 or more, but some cardiomyocytes beat 3 times faster than normal at 7.5-20 micromol x L(-1) AgNO3. Picomolar Ag+ increases marine egg polar lobe constriction within an hour, even in the absence of microtubules. CONCLUSION: Ag+ alters animal cell growth and shape changes by a MT-independent mechanism. This is the first report of Ag+ effects on vertebrate neurite outgrowth, glial cell migration, or cardiomyocyte beat rate. |
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Authors:
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A H Conrad; C R Tramp; C J Long; D C Wells; A Q Paulsen; G W Conrad |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Aviation, space, and environmental medicine Volume: 70 ISSN: 0095-6562 ISO Abbreviation: Aviat Space Environ Med Publication Date: 1999 Nov |
Date Detail:
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Created Date: 2000-01-07 Completed Date: 2000-01-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7501714 Medline TA: Aviat Space Environ Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1096-105 Citation Subset: IM; S |
Affiliation:
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Division of Biology, Kansas State University, Manhattan 66506-4901, USA. aconrad@ksu.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Division / drug effects* Cells, Cultured / drug effects Chick Embryo Drug Evaluation, Preclinical Ecological Systems, Closed* Epithelial Cells / drug effects Humans Intestinal Mucosa / cytology Intestine, Small / cytology Mice Myocardial Contraction / drug effects*, physiology Myocardium / cytology* Neurites / drug effects*, physiology Rats Silver Nitrate / adverse effects* Space Flight* Spacecraft* Water Purification / methods* Zygote / drug effects*, physiology |
| Chemical | |
Reg. No./Substance:
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7761-88-8/Silver Nitrate |
| Investigator | |
Investigator/Affiliation:
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B S Spooner / KS St U, Manhattan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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