Document Detail

The Ag-NOR proteins present a crescent-shaped distribution at the secondary constrictions of metaphase PtK1 chromosomes.
MedLine Citation:
PMID:  9040783     Owner:  NLM     Status:  MEDLINE    
The ultrastructural distribution of the proteins responsible for the silver staining of NORs was analyzed on serial thin sections of preembedded silver-stained rat kangaroo PtK1 metaphase chromosomes. Our results show that the Ag-NOR proteins present a crescent-shaped distribution at the secondary constriction of each chromatid. Moreover, in some cases the crescent-shaped structures of both chromatids are not symmetrically arranged but show different orientations. These observations, together with our own and previously reported light microscopy results obtained on silver-stained mammalian chromosomes, lead us to suggest a mechanism for the formation of apparent secondary constrictions at NORs.
J A Suja; D Hernandez-Verdun
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cytogenetics and cell genetics     Volume:  75     ISSN:  0301-0171     ISO Abbreviation:  Cytogenet. Cell Genet.     Publication Date:  1996  
Date Detail:
Created Date:  1997-03-13     Completed Date:  1997-03-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0367735     Medline TA:  Cytogenet Cell Genet     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  155-8     Citation Subset:  IM    
Departamento de Biología, Universidad Autónoma de Madrid, Spain. jasuja@ccuam3.sdi.uam.cs
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MeSH Terms
Azure Stains
Cell Line
Chromatids / chemistry,  ultrastructure
Chromosome Banding
Chromosomes / chemistry*,  ultrastructure
Microscopy, Electron
Nuclear Proteins / analysis*
Nucleolus Organizer Region / chemistry*,  ultrastructure
Silver Staining
X Chromosome / chemistry,  ultrastructure
Reg. No./Substance:
0/Azure Stains; 0/Nuclear Proteins

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