Document Detail


African trypanosomes express an immunogenic protein with a repeating epitope of 24 amino acids.
MedLine Citation:
PMID:  1723507     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Infection with intracellular protozoan parasites such as Plasmodium, Leishmania and Trypanosoma cruzi induces a strong antibody response against proteins containing tandem repeats, suggesting that these repetitive epitopes may camouflage vulnerable parasite antigens from a 'protective' immune response. We tested this theory by immunoscreening a cDNA expression library of African trypanosomes, extracellular parasites that evade their hosts' immune response by antigenic variation, and found that the most frequently detected trypanosome protein contains more than 40 tandem copies of a 24-amino acid repeat with a consensus sequence of A-M-E-D-E-L-D-S-L-R-A-L-N-E-Q-Y-E-A-L-Q-R-T-N-A (net charge = -4). This protein is encoded on an mRNA of more than 20 kb and has slight sequence similarities with cytoskeletal, intermediate filament proteins in other organisms. Thus, protozoan proteins with tandemly repeating epitopes do not exist solely to divert the humoral immune response; they have other specific physiological functions for the parasites and affect the overall parasite-host interaction in unknown and perhaps different ways.
Authors:
L R Duncan; L S Gay; J E Donelson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and biochemical parasitology     Volume:  48     ISSN:  0166-6851     ISO Abbreviation:  Mol. Biochem. Parasitol.     Publication Date:  1991 Sep 
Date Detail:
Created Date:  1992-03-09     Completed Date:  1992-03-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8006324     Medline TA:  Mol Biochem Parasitol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  11-6     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of Iowa, Iowa City 52242.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/M60557;  M60558;  M61205;  M61206;  M61207;  M61208;  S43024;  S78950;  S79890;  S79892
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Antigens, Protozoan / genetics*
Base Sequence
DNA / genetics
Epitopes / genetics
Mice
Molecular Sequence Data
Protozoan Proteins / genetics,  immunology*
Repetitive Sequences, Nucleic Acid
Trypanosoma brucei gambiense / genetics,  immunology*
Grant Support
ID/Acronym/Agency:
AI 18954/AI/NIAID NIH HHS; DK 25295/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, Protozoan; 0/Epitopes; 0/Protozoan Proteins; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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