Document Detail


Affinity and rate constants for interactions of bovine folate-binding protein and folate derivatives determined by optical biosensor technology. Effect of stereoselectivity.
MedLine Citation:
PMID:  15969536     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The interactions between bovine folate-binding protein (FBP) and different folate derivatives in pure diastereoisomeric forms were studied at pH 7.4 by a surface plasmon resonance technology (Biacore). The results show that folic acid had the most rapid association rate (k(a) = 1.0 x 10(6) M(-)(1) s(-)(1)), whereas (6S)-5-HCO-5,6,7,8-tetrahydrofolic acid had the most rapid dissociation rate (k(d) = 3.2 x l0(-)(3) s(-)(1)). The equilibrium dissociation constant (K(D)), calculated from the quotient of k(d)/k(a), showed that the two forms of folates not occurring in nature, that is, folic acid and (6R)-5-CH(3)-5,6,7,8-tetrahydrofolic acid, had the highest affinities for FBP, 20 and 160 pmol/L, respectively. The results thus show that there were great differences in the interactions between folate-binding protein and the major forms of folate derivatives. The nutritional implications of these differences are discussed.
Authors:
Linnéa Nygren-Babol; Ase Sternesjö; Margareta Jägerstad; Lennart Björck
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  53     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-06-22     Completed Date:  2005-08-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5473-8     Citation Subset:  IM    
Affiliation:
Department of Food Science, Swedish University of Agricultural Sciences, Uppsala.
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MeSH Terms
Descriptor/Qualifier:
Carrier Proteins / metabolism*
Folic Acid / analogs & derivatives*,  metabolism
Hydrogen-Ion Concentration
Kinetics
Receptors, Cell Surface / metabolism*
Stereoisomerism
Surface Plasmon Resonance
Tetrahydrofolates / metabolism
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Receptors, Cell Surface; 0/Tetrahydrofolates; 0/folate-binding protein; 134-35-0/5-methyltetrahydrofolate; 59-30-3/Folic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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