| Affinity and rate constants for interactions of bovine folate-binding protein and folate derivatives determined by optical biosensor technology. Effect of stereoselectivity. | |
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MedLine Citation:
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PMID: 15969536 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The interactions between bovine folate-binding protein (FBP) and different folate derivatives in pure diastereoisomeric forms were studied at pH 7.4 by a surface plasmon resonance technology (Biacore). The results show that folic acid had the most rapid association rate (k(a) = 1.0 x 10(6) M(-)(1) s(-)(1)), whereas (6S)-5-HCO-5,6,7,8-tetrahydrofolic acid had the most rapid dissociation rate (k(d) = 3.2 x l0(-)(3) s(-)(1)). The equilibrium dissociation constant (K(D)), calculated from the quotient of k(d)/k(a), showed that the two forms of folates not occurring in nature, that is, folic acid and (6R)-5-CH(3)-5,6,7,8-tetrahydrofolic acid, had the highest affinities for FBP, 20 and 160 pmol/L, respectively. The results thus show that there were great differences in the interactions between folate-binding protein and the major forms of folate derivatives. The nutritional implications of these differences are discussed. |
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Authors:
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Linnéa Nygren-Babol; Ase Sternesjö; Margareta Jägerstad; Lennart Björck |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 53 ISSN: 0021-8561 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2005 Jun |
Date Detail:
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Created Date: 2005-06-22 Completed Date: 2005-08-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 5473-8 Citation Subset: IM |
Affiliation:
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Department of Food Science, Swedish University of Agricultural Sciences, Uppsala. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Carrier Proteins
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metabolism* Folic Acid / analogs & derivatives*, metabolism Hydrogen-Ion Concentration Kinetics Receptors, Cell Surface / metabolism* Stereoisomerism Surface Plasmon Resonance Tetrahydrofolates / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Receptors, Cell Surface; 0/Tetrahydrofolates; 0/folate-binding protein; 134-35-0/5-methyltetrahydrofolate; 59-30-3/Folic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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