| Affinity purification of the major bovine allergen by a novel monoclonal antibody. | |
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MedLine Citation:
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PMID: 8182227 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A monoclonal antibody was developed to the 20 kd major allergen of cow by immunizing mice with crude dander extract. The monoclonal antibody did not exhibit cross-reactivity to cat, dog, and horse dander extracts when studied by ELISA inhibition. The antibody was used in affinity chromatography for the purification of the 20 kd allergen from cow dander extract. Purity of the allergen was estimated to be 88%, and allergenic reactivity was verified by IgE immunoblotting and skin prick tests. After further purification with size-exclusion chromatography, the allergen was almost 100% pure. The isoelectric point of the double-purified allergen was determined to be 4.1. The amino acid composition was characterized by the predominance of acidic amino acids. |
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Authors:
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J Ylönen; T Virtanen; L Horsmanheimo; S Parkkinen; J Pelkonen; R Mäntyjärvi |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of allergy and clinical immunology Volume: 93 ISSN: 0091-6749 ISO Abbreviation: J. Allergy Clin. Immunol. Publication Date: 1994 May |
Date Detail:
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Created Date: 1994-06-16 Completed Date: 1994-06-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1275002 Medline TA: J Allergy Clin Immunol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 851-8 Citation Subset: AIM; IM |
Affiliation:
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Department of Clinical Microbiology, University of Kuopio, Finland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Allergens / analysis, immunology, isolation & purification* Animals Antibodies, Monoclonal* / isolation & purification Cats Cattle Chromatography, Affinity Cross Reactions Dogs Epithelium / immunology Female Horses Humans Immunization Immunologic Techniques Male Mice Mice, Inbred BALB C Middle Aged Molecular Weight Skin Tests |
| Chemical | |
Reg. No./Substance:
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0/Allergens; 0/Antibodies, Monoclonal |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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