Document Detail

Affinity purification of antibodies specific for Asn-linked glycans containing alpha 1-->3 fucose or beta 1-->2 xylose.
MedLine Citation:
PMID:  7682035     Owner:  NLM     Status:  MEDLINE    
Antisera raised against the plant glycoproteins beta-fructosidase and horseradish peroxidase can be fractionated on an affinity column of honeybee venom phospholipase A2 to produce serum fractions that are specific for either the alpha 1-->3 fucose or beta 1-->2 xylose epitopes commonly found on the Asn-linked glycans of plant glycoproteins. This affinity purification strategy relies on the absence of beta 1-->2 xylose from the glycan of the venom protein. Such antibody preparations can be used for the detection of these sugar epitopes on glycoproteins.
L Faye; V Gomord; A C Fitchette-Lainé; M J Chrispeels
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Analytical biochemistry     Volume:  209     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1993 Feb 
Date Detail:
Created Date:  1993-05-03     Completed Date:  1993-05-03     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  104-8     Citation Subset:  IM    
European Institute for Peptide Research, LTI-CNRS URA 203, University of Rouen, Mont Saint Aignan, France.
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MeSH Terms
Antibodies / immunology,  isolation & purification*
Antibody Specificity
Asparagine / analysis*
Bee Venoms / enzymology
Carbohydrate Sequence
Chromatography, Affinity / methods
Enzymes, Immobilized
Epitopes / analysis
Fucose / analysis,  immunology*
Glycoproteins / immunology
Glycoside Hydrolases / immunology
Horseradish Peroxidase / immunology
Molecular Sequence Data
Phospholipases A
Phospholipases A2
Plant Proteins / immunology
Polysaccharides / analysis,  immunology*
Xylose / analysis,  immunology*
Reg. No./Substance:
0/Antibodies; 0/Bee Venoms; 0/Enzymes, Immobilized; 0/Epitopes; 0/Glycoproteins; 0/Plant Proteins; 0/Polysaccharides; 0/Xylose; 3713-31-3/Fucose; 7006-34-0/Asparagine; EC 1.11.1.-/Horseradish Peroxidase; EC 3.1.1.-/Phospholipases A; EC A2; EC 3.2.1.-/Glycoside Hydrolases; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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