Document Detail

Affinity modification of creatine kinase and ATP-ADP translocase in heart mitochondria: determination of their molar stoichiometry.
MedLine Citation:
PMID:  3004438     Owner:  NLM     Status:  MEDLINE    
Oxidized dialdehyde analogs of ADP or ATP (oADP and oATP) were shown to inhibit irreversibly adenine nucleotide translocator (T) and creatine kinase (CK) in heart mitochondria. Inactivation of T and CK was parallel with carboxyatractyloside - sensitive and (ADP + phosphocreatine) - sensitive incorporation of o[3H]ADP into mitochondria, respectively. o[3H]ADP incorporation sensitive to CAT or ADP+phosphocreatine was used to determine T and CK contents in mitochondria. T content in cardiac mitochondria from rat, rabbit, dog, and chicken was calculated to be 2.6 - 2.9 moles/mole cyt.aa3. The same value of T/cyt.aa3 ratio was found in liver mitochondria with lower cytochrome aa3 content. In all types of cardiac mitochondria CK content was found to be 2.4 - 2.6 moles/mole cyt.aa3. The data show that T and CK are present in molar ratio 1:1 in all types of cardiac mitochondria.
A V Kuznetsov; V A Saks
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  134     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1986 Jan 
Date Detail:
Created Date:  1986-03-14     Completed Date:  1986-03-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  359-66     Citation Subset:  IM    
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MeSH Terms
Adenosine Diphosphate / analogs & derivatives,  metabolism
Adenosine Triphosphate / analogs & derivatives,  metabolism
Atractyloside / analogs & derivatives,  pharmacology
Carrier Proteins / metabolism
Creatine Kinase / metabolism*
Mitochondria, Heart / enzymology*
Mitochondrial ADP, ATP Translocases / metabolism*
Nucleotidyltransferases / metabolism*
Reg. No./Substance:
0/Carrier Proteins; 17754-44-8/Atractyloside; 35988-42-2/carboxyatractyloside; 54970-91-1/2',3'-dialdehyde ATP; 56-65-5/Adenosine Triphosphate; 58-64-0/Adenosine Diphosphate; 64060-84-0/adenosine 5'-diphosphate 2',3'-dialdehyde; 9068-80-8/Mitochondrial ADP, ATP Translocases; EC Kinase; EC 2.7.7.-/Nucleotidyltransferases

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