| Affinity modification of creatine kinase and ATP-ADP translocase in heart mitochondria: determination of their molar stoichiometry. | |
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MedLine Citation:
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PMID: 3004438 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Oxidized dialdehyde analogs of ADP or ATP (oADP and oATP) were shown to inhibit irreversibly adenine nucleotide translocator (T) and creatine kinase (CK) in heart mitochondria. Inactivation of T and CK was parallel with carboxyatractyloside - sensitive and (ADP + phosphocreatine) - sensitive incorporation of o[3H]ADP into mitochondria, respectively. o[3H]ADP incorporation sensitive to CAT or ADP+phosphocreatine was used to determine T and CK contents in mitochondria. T content in cardiac mitochondria from rat, rabbit, dog, and chicken was calculated to be 2.6 - 2.9 moles/mole cyt.aa3. The same value of T/cyt.aa3 ratio was found in liver mitochondria with lower cytochrome aa3 content. In all types of cardiac mitochondria CK content was found to be 2.4 - 2.6 moles/mole cyt.aa3. The data show that T and CK are present in molar ratio 1:1 in all types of cardiac mitochondria. |
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Authors:
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A V Kuznetsov; V A Saks |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 134 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1986 Jan |
Date Detail:
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Created Date: 1986-03-14 Completed Date: 1986-03-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 359-66 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Diphosphate
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analogs & derivatives,
metabolism Adenosine Triphosphate / analogs & derivatives, metabolism Animals Atractyloside / analogs & derivatives, pharmacology Carrier Proteins / metabolism Chickens Creatine Kinase / metabolism* Dogs Kinetics Mitochondria, Heart / enzymology* Mitochondrial ADP, ATP Translocases / metabolism* Nucleotidyltransferases / metabolism* Rabbits Rats |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 17754-44-8/Atractyloside; 35988-42-2/carboxyatractyloside; 54970-91-1/2',3'-dialdehyde ATP; 56-65-5/Adenosine Triphosphate; 58-64-0/Adenosine Diphosphate; 64060-84-0/adenosine 5'-diphosphate 2',3'-dialdehyde; 9068-80-8/Mitochondrial ADP, ATP Translocases; EC 2.7.3.2/Creatine Kinase; EC 2.7.7.-/Nucleotidyltransferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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