| Affinity labeling of D-amino acid oxidase with an acetylenic substrate. | |
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MedLine Citation:
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PMID: 379 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The acetylenic substrate, D-2-amino-4-pentynoic acid (D-propargylglycine), was oxidatively deaminated by hog kidney D-amino acid oxidase[EC 1.4.3.3], with accompanying inactivation of the enzyme. The flavin which was extracted by hot methanol from the inactivated enzyme was identical with authentic FAD by thin-layer chromatography and circular dichroism. The excitation spectrum of emission at 520 nm of the released flavin was very similar to the absorption spectrum of oxidized FAD. The released flavin was reduced by potassium borohydride. The apoenzyme prepared after propargylglycine treatment did not show restored D-amino acid oxidase activity on adding exogenous FAD. The absorption spectrum of this inactivated apoenzyme showed absorption peaks at 279 and 317 nm, and a shoulder at about 290 nm. These results strongly indicate that the inactivation reaction is a dynamic affinity labeling with D-propargylglycine which produces irreversible inactivation of the enzyme by a covalent modification of an amino acid residue at the active site. |
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Authors:
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K Horiike; Y Nishina; Y Miyake; T Yamano |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of biochemistry Volume: 78 ISSN: 0021-924X ISO Abbreviation: J. Biochem. Publication Date: 1975 Jul |
Date Detail:
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Created Date: 1976-02-21 Completed Date: 1976-02-21 Revised Date: 2007-12-19 |
Medline Journal Info:
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Nlm Unique ID: 0376600 Medline TA: J Biochem Country: JAPAN |
Other Details:
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Languages: eng Pagination: 57-63 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Affinity Labels Alkynes / metabolism* Amino Acids / metabolism* Animals Catalase / metabolism Circular Dichroism D-Amino-Acid Oxidase / metabolism* Deamination Enzyme Activation Flavin-Adenine Dinucleotide / metabolism Kidney / enzymology Oxygen Consumption Swine |
| Chemical | |
Reg. No./Substance:
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0/Affinity Labels; 0/Alkynes; 0/Amino Acids; 146-14-5/Flavin-Adenine Dinucleotide; EC 1.11.1.6/Catalase; EC 1.4.3.3/D-Amino-Acid Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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