Document Detail


Affinity chromatography purification of Pseudomonas aeruginosa exotoxin A on specifically linked NAD agarose.
MedLine Citation:
PMID:  7061386     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The specific binding of P. aeruginosa exotoxin A to NAD was exploited for the rapid purification of the toxin. Affinity chromatography on a column of agarose-N6-(aminohexyl)carbamoylmethyl-NAD resulted in an enzymatically, biologically, and immunologically active purified toxin preparation. Other NAD-agarose resins were not efficient substrates for toxin purification.
Authors:
G Cukor; N A Nowak
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of bacteriology     Volume:  149     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1982 Mar 
Date Detail:
Created Date:  1982-05-21     Completed Date:  1982-05-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1162-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
ADP Ribose Transferases*
Bacterial Toxins*
Chromatography, Affinity
Exotoxins / isolation & purification*,  metabolism,  pharmacology
NAD / metabolism
Sepharose
Virulence Factors*
Chemical
Reg. No./Substance:
0/Bacterial Toxins; 0/Exotoxins; 0/Virulence Factors; 53-84-9/NAD; 9012-36-6/Sepharose; EC 2.4.2.-/ADP Ribose Transferases; EC 2.4.2.31/toxA protein, Pseudomonas aeruginosa
Comments/Corrections

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