Document Detail


Affinity chromatographic studies on antigen-antibody dissociation.
MedLine Citation:
PMID:  6437868     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An analytical affinity chromatography assay has been developed for the investigation of the dissociation of antigen-antibody complexes. Albumin-coupled Sepharose 4B and anti-albumin has been used as a model system. At extremely low or high pH, in the presence of highly concentrated chaotropic ions at pH 7 or by elution with 100% ethylene glycol after pretreating with high pH buffer, most of the bondings could be ruptured. The latter two-step desorption procedure provides recovery of intact antibody with high yield. The technique was also utilized for the preparation of antibody against human growth hormone.
Authors:
N Fornstedt
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEBS letters     Volume:  177     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1984 Nov 
Date Detail:
Created Date:  1985-01-09     Completed Date:  1985-01-09     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  195-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Antigen-Antibody Complex / analysis*
Chromatography, Affinity / methods
Growth Hormone / immunology
Humans
Immunodiffusion
Serum Albumin / analysis,  immunology
Chemical
Reg. No./Substance:
0/Antigen-Antibody Complex; 0/Serum Albumin; 9002-72-6/Growth Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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