| Affinity of chicken cystatin for S-(carbamoylmethyl)-papain, measured by fluorescence at acidic pH. | |
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MedLine Citation:
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PMID: 12144519 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have characterized the interaction between S-(carbamoylmethyl)-papain and chicken cystatin by means of fluorescence titration at acidic pH. The calculated binding constant was 32 x 10(6), which is less than the tight affinity at pH 7.4 (approximately 7 x 10(10)). Results have been discussed in terms of the burial of Trp-104 of cystatin and the possible role of the charge of the carbamoylmethyl moiety. |
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Authors:
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Alfonso Arroyo-Reyna; Ezequiel Gutiérrez-Gómez |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Protein and peptide letters Volume: 9 ISSN: 0929-8665 ISO Abbreviation: Protein Pept. Lett. Publication Date: 2002 Jun |
Date Detail:
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Created Date: 2002-07-29 Completed Date: 2002-11-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9441434 Medline TA: Protein Pept Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 231-5 Citation Subset: IM |
Affiliation:
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Area de Biofisicoquímica, Departamento de Química, Universidad Autónoma Metropolitana-Iztapalapa, Apartado Postal, México. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chickens Cystatins / chemistry* Dose-Response Relationship, Drug Humans Hydrogen-Ion Concentration Papain / chemistry* Spectrometry, Fluorescence Temperature Tryptophan / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Cystatins; 73-22-3/Tryptophan; EC 3.4.22.-/S-carboxymethyl-papain; EC 3.4.22.2/Papain |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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