Document Detail


Afcwh41 is required for cell wall synthesis, conidiation, and polarity in Aspergillus fumigatus.
MedLine Citation:
PMID:  19090038     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
alpha-Glucosidase I regulates trimming of the terminal alpha-1,2-glucose residue in the N-glycan-processing pathway, which plays an important role in the quality control system in mammalian cells. However, the consequence of glucose trimming of the N-glycan in filamentous fungi is unclear. We identified the gene encoding alpha-glucosidase I in the human opportunistic fungal pathogen Aspergillus fumigatus, namely Afcwh41. Deletion of the Afcwh41 gene resulted in a defective N-glycan processing of the proteins secreted by A. fumigatus. Although the Afcwh41 was not essential for hyphal growth and virulence, a severe reduction in conidia formation and a temperature-sensitive deficiency of cell wall integrity (CWI) were observed. Also, abnormalities of polar growth and septation were observed during conidial germination and hyphal elongation of the mutant. Our results suggest that Afcwh41 was involved in CWI, polarity, septation, and conidiation in A. fumigatus, probably by affecting the proper function of the proteins that are required for cell wall synthesis.
Authors:
Lei Zhang; Hui Zhou; Haomiao Ouyang; Yanjie Li; Cheng Jin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS microbiology letters     Volume:  289     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-12-16     Completed Date:  2009-01-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  England    
Other Details:
Languages:  eng     Pagination:  155-65     Citation Subset:  IM    
Affiliation:
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aspergillosis / microbiology*
Aspergillus fumigatus / cytology*,  genetics,  metabolism*,  pathogenicity
Cell Wall / genetics,  metabolism*
Fungal Proteins / genetics,  metabolism*
Gene Expression Regulation, Fungal
Humans
Male
Mice
Mice, Inbred BALB C
Spores, Fungal / genetics,  metabolism*
Virulence
Chemical
Reg. No./Substance:
0/Fungal Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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