Document Detail


Aerosolized ZnO nanoparticles induce toxicity in alveolar type II epithelial cells at the air-liquid interface.
MedLine Citation:
PMID:  21964423     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The majority of in vitro studies characterizing the impact of engineered nanoparticles (NPs) on cells that line the respiratory tract were conducted in cells exposed to NPs in suspension. This approach introduces processes that are unlikely to occur during inhaled NP exposures in vivo, such as the shedding of toxic doses of dissolved ions. ZnO NPs are used extensively and pose significant sources for human exposure. Exposures to airborne ZnO NPs can induce adverse effects, but the relevance of the dissolved Zn(2+) to the observed effects in vivo is still unclear. Our goal was to mimic in vivo exposures to airborne NPs and decipher the contribution of the intact NP from the contribution of the dissolved ions to airborne ZnO NP toxicity. We established the exposure of alveolar type II epithelial cells to aerosolized NPs at the air-liquid interface (ALI) and compared the impact of aerosolized ZnO NPs and NPs in suspension at the same cellular doses, measured as the number of particles per cell. By evaluating membrane integrity and cell viability 6 and 24 h post-exposure, we found that aerosolized NPs induced toxicity at the ALI at doses that were in the same order of magnitude as doses required to induce toxicity in submersed cultures. In addition, distinct patterns of oxidative stress were observed in the two exposure systems. These observations unravel the ability of airborne ZnO NPs to induce toxicity without the contribution of dissolved Zn(2+) and suggest distinct mechanisms at the ALI and in submersed cultures.
Authors:
Yumei Xie; Nolann G Williams; Ana Tolic; William B Chrisler; Justin G Teeguarden; Bettye L S Maddux; Joel G Pounds; Alexander Laskin; Galya Orr
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-09-28
Journal Detail:
Title:  Toxicological sciences : an official journal of the Society of Toxicology     Volume:  125     ISSN:  1096-0929     ISO Abbreviation:  Toxicol. Sci.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-01-23     Completed Date:  2012-05-17     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  9805461     Medline TA:  Toxicol Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  450-61     Citation Subset:  IM    
Affiliation:
Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99352, USA.
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MeSH Terms
Descriptor/Qualifier:
Aerosols
Animals
Blood-Air Barrier / drug effects*,  metabolism,  pathology
Cell Culture Techniques
Cell Line
Cell Proliferation / drug effects
Cell Survival / drug effects
Dose-Response Relationship, Drug
Mice
Nanoparticles*
Oxidative Stress / drug effects
Particle Size
Pneumocytes / drug effects*,  metabolism,  pathology
Pulmonary Alveoli / drug effects*,  metabolism,  pathology
Time Factors
Zinc Oxide / toxicity*
Grant Support
ID/Acronym/Agency:
1RC2ES018786-01/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Aerosols; 1314-13-2/Zinc Oxide
Comments/Corrections

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