Document Detail


Aerobic growth of Escherichia coli with 2,4,6-trinitrotoluene (TNT) as the sole nitrogen source and evidence of TNT denitration by whole cells and cell-free extracts.
MedLine Citation:
PMID:  17012591     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Escherichia coli grew aerobically with 2,4,6-trinitrotoluene (TNT) as sole nitrogen source and caused TNT's partial denitration. This reaction was enhanced in nongrowing cell suspensions with 0.516 mol nitrite released per mol TNT. Cell extracts denitrated TNT in the presence of NAD(P)H. Isomers of amino-dimethyl-tetranitrobiphenyl were detected and confirmed with U-15N-labeled TNT.
Authors:
Ben Stenuit; Laurent Eyers; Raoul Rozenberg; Jean-Louis Habib-Jiwan; Spiros N Agathos
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-09-29
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  72     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-12-06     Completed Date:  2007-01-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7945-8     Citation Subset:  IM    
Affiliation:
Unit of Bioengineering, Department of Applied Chemistry and Bioindustries, Catholic University of Lovain, Place Croix du Sud 2/19, 1348 Louvain-la-Neuve, Belgium.
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MeSH Terms
Descriptor/Qualifier:
Aerobiosis
Biodegradation, Environmental
Culture Media
Escherichia coli / cytology,  growth & development*,  metabolism
Kinetics
Nitrites / metabolism
Nitrogen
Trinitrotoluene / metabolism*
Chemical
Reg. No./Substance:
0/Culture Media; 0/Nitrites; 118-96-7/Trinitrotoluene; 7727-37-9/Nitrogen
Comments/Corrections

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