Document Detail


Aerobic expression of the cyf gene encoding cytochrome c-553 from Desulfovibrio vulgaris Hildenborough in Escherichia coli.
MedLine Citation:
PMID:  8012605     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Monohaem cytochrome c-553 from Desulfovibrio vulgaris Hildenborough is encoded by the cyf gene which could be expressed in Escherichia coli to yield the periplasmic holoform of cytochrome c-553. Covalent haem attachment was shown by labelling with 5-amino[4-14C]laevulinic acid, the immediate precursor for haem biosynthesis. Visible-absorption spectroscopy demonstrated that the haem environment in the recombinant protein did not differ from the native protein. Optimal expression was obtained under aerobic conditions. Furthermore, efficient insertion of haem into a cytochrome c-553-beta-lactamase fusion protein could be demonstrated. We suggest that the varying success in heterologous expression of c-type cytochromes in E. coli may arise as a result of differences in the physical properties of the apoproteins.
Authors:
W B Pollock; G Voordouw
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  140 ( Pt 4)     ISSN:  1350-0872     ISO Abbreviation:  Microbiology (Reading, Engl.)     Publication Date:  1994 Apr 
Date Detail:
Created Date:  1994-07-25     Completed Date:  1994-07-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  879-87     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, University of Calgary, Alberta, Canada.
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MeSH Terms
Descriptor/Qualifier:
Aerobiosis
Anaerobiosis
Bacterial Proteins / biosynthesis*,  genetics
Cytochrome c Group / biosynthesis*,  genetics
Desulfovibrio vulgaris / enzymology,  genetics*
Enzyme Induction
Escherichia coli
Genes, Bacterial*
Heme / metabolism
Recombinant Fusion Proteins / biosynthesis*,  genetics
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Cytochrome c Group; 0/Recombinant Fusion Proteins; 0/cytochrome c553; 14875-96-8/Heme; 9048-78-6/cytochrome C-552

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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