| Aerobic expression of the cyf gene encoding cytochrome c-553 from Desulfovibrio vulgaris Hildenborough in Escherichia coli. | |
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MedLine Citation:
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PMID: 8012605 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Monohaem cytochrome c-553 from Desulfovibrio vulgaris Hildenborough is encoded by the cyf gene which could be expressed in Escherichia coli to yield the periplasmic holoform of cytochrome c-553. Covalent haem attachment was shown by labelling with 5-amino[4-14C]laevulinic acid, the immediate precursor for haem biosynthesis. Visible-absorption spectroscopy demonstrated that the haem environment in the recombinant protein did not differ from the native protein. Optimal expression was obtained under aerobic conditions. Furthermore, efficient insertion of haem into a cytochrome c-553-beta-lactamase fusion protein could be demonstrated. We suggest that the varying success in heterologous expression of c-type cytochromes in E. coli may arise as a result of differences in the physical properties of the apoproteins. |
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Authors:
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W B Pollock; G Voordouw |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Microbiology (Reading, England) Volume: 140 ( Pt 4) ISSN: 1350-0872 ISO Abbreviation: Microbiology (Reading, Engl.) Publication Date: 1994 Apr |
Date Detail:
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Created Date: 1994-07-25 Completed Date: 1994-07-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9430468 Medline TA: Microbiology Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 879-87 Citation Subset: IM |
Affiliation:
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Department of Biological Sciences, University of Calgary, Alberta, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aerobiosis Anaerobiosis Bacterial Proteins / biosynthesis*, genetics Cytochrome c Group / biosynthesis*, genetics Desulfovibrio vulgaris / enzymology, genetics* Enzyme Induction Escherichia coli Genes, Bacterial* Heme / metabolism Recombinant Fusion Proteins / biosynthesis*, genetics |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Cytochrome c Group; 0/Recombinant Fusion Proteins; 0/cytochrome c553; 14875-96-8/Heme; 9048-78-6/cytochrome C-552 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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