| Aerobic exercise training improves Ca2+ handling and redox status of skeletal muscle in mice. | |
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MedLine Citation:
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PMID: 20407082 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Exercise training is known to promote relevant changes in the properties of skeletal muscle contractility toward powerful fibers. However, there are few studies showing the effect of a well-established exercise training protocol on Ca(2+) handling and redox status in skeletal muscles with different fiber-type compositions. We have previously standardized a valid and reliable protocol to improve endurance exercise capacity in mice based on maximal lactate steady-state workload (MLSSw). The aim of this study was to investigate the effect of exercise training, performed at MLSSw, on the skeletal muscle Ca(2+) handling-related protein levels and cellular redox status in soleus and plantaris. Male C57BL/6J mice performed treadmill training at MLSSw over a period of eight weeks. Muscle fiber-typing was determined by myosin ATPase histochemistry, citrate synthase activity by spectrophotometric assay, Ca(2+) handling-related protein levels by Western blot and reduced to oxidized glutathione ratio (GSH:GSSG) by high-performance liquid chromatography. Trained mice displayed higher running performance and citrate synthase activity compared with untrained mice. Improved running performance in trained mice was paralleled by fast-to-slow fiber-type shift and increased capillary density in both plantaris and soleus. Exercise training increased dihydropyridine receptor (DHPR) alpha2 subunit, ryanodine receptor and Na(+)/Ca(2+) exchanger levels in plantaris and soleus. Moreover, exercise training elevated DHPR beta1 subunit and sarcoplasmic reticulum Ca(2+)-ATPase (SERCA) 1 levels in plantaris and SERCA2 levels in soleus of trained mice. Skeletal muscle GSH content and GSH:GSSG ratio was increased in plantaris and soleus of trained mice. Taken together, our findings indicate that MLSSw exercise-induced better running performance is, in part, due to increased levels of proteins involved in skeletal muscle Ca(2+) handling, whereas this response is partially dependent on specificity of skeletal muscle fiber-type composition. Finally, we demonstrated an augmented cellular redox status and GSH antioxidant capacity in trained mice. |
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Authors:
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Julio C B Ferreira; Aline V Bacurau; Carlos R Bueno; Telma C Cunha; Leonardo Y Tanaka; Maira A Jardim; Paulo R Ramires; Patricia C Brum |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental biology and medicine (Maywood, N.J.) Volume: 235 ISSN: 1535-3699 ISO Abbreviation: Exp. Biol. Med. (Maywood) Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-04-21 Completed Date: 2010-04-28 Revised Date: 2010-07-29 |
Medline Journal Info:
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Nlm Unique ID: 100973463 Medline TA: Exp Biol Med (Maywood) Country: England |
Other Details:
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Languages: eng Pagination: 497-505 Citation Subset: IM |
Affiliation:
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School of Physical Education and Sport, University of Sao Paulo (USP), São Paulo 05508-900, Brazil. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Absorptiometry, Photon Animals Body Composition Calcium / metabolism* Lipid Peroxides / metabolism Male Mice Mice, Inbred C57BL Muscle, Skeletal / physiology* Oxidation-Reduction Oxidative Stress Physical Conditioning, Animal / physiology* Random Allocation Running / physiology* Sarcoplasmic Reticulum / metabolism Sarcoplasmic Reticulum Calcium-Transporting ATPases / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Lipid Peroxides; 7440-70-2/Calcium; EC 3.6.3.8/Sarcoplasmic Reticulum Calcium-Transporting ATPases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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