Document Detail

Aerobic exercise's reversal of insulin resistance by activating AMPKα-ACC-CPT1 signaling in the skeletal muscle of C57BL/6 mice.
MedLine Citation:
PMID:  20975105     Owner:  NLM     Status:  MEDLINE    
Insulin resistance (IR) is a common pathophysiological feature of Type 2 diabetes. Although the mechanisms leading to IR are still elusive, evidence has shown that aerobic exercise can reverse this process. To investigate the effects of aerobic exercise on IR, the authors created an IR animal model by feeding C57BL/6 mice a high-fat diet for 8 wk. They then compared the effect of 6 wk of treadmill training (60 min/d) at 75% VO2max on mice in normal-diet (NE) and high-fat-diet (HE) groups with their sedentary control groups. Levels of skeletal-muscle AMPKα (AMP-activated protein kinase α), ACC (acetyl-CoA carboxylases), and CPT1 (carnitine palmitoyltransferase 1) mRNA and AMPKα, pAMPK-Thr172, ACC, pACC-Ser79, and CPT1 protein expressions were analyzed. In addition, fasting serum levels of insulin, triglyceride, and cholesterol were measured. The results demonstrate that 6 wk of exercise increased AMPKα mRNA expression by 11% and 25 % (p<.01) in the NE and HE groups, respectively, and AMPKα protein expression by 37.9% and 20.1% (p<.01) in NE and HE compared with their sedentary control. In addition, ACC mRNA and protein expressions declined, whereas CPT1 mRNA and protein expressions were elevated in both exercise groups compared with sedentary control groups. In addition, pAMPK-Thr172 and pACC-Ser79 expression increased significantly in the NE and HE groups compared with sedentary control groups. In conclusion, our results demonstrate that 6 wk of aerobic exercise can effectively ameliorate IR by increasing the expression of AMPKα and pAMPK-Thr172, thereby activating the key enzymes that facilitate lipid metabolism.
Yanmei Niu; Hong Yuan; Li Fu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of sport nutrition and exercise metabolism     Volume:  20     ISSN:  1526-484X     ISO Abbreviation:  Int J Sport Nutr Exerc Metab     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-26     Completed Date:  2010-11-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100939812     Medline TA:  Int J Sport Nutr Exerc Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  370-80     Citation Subset:  IM    
Department of Health and Exercise Science, Tianjin Institute of Physical Education, Tianjin, China.
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MeSH Terms
AMP-Activated Protein Kinases / metabolism*
Acetyl-CoA Carboxylase / metabolism
Area Under Curve
Blood Glucose / metabolism
Carnitine O-Palmitoyltransferase / metabolism
Dietary Fats / pharmacology*
Gene Expression Regulation, Enzymologic / drug effects*
Glucose Tolerance Test
Insulin / blood
Insulin Resistance*
Lipid Metabolism / drug effects
Mice, Inbred C57BL
Models, Animal
Muscle, Skeletal / metabolism*
RNA, Messenger / analysis
Signal Transduction
Reg. No./Substance:
0/Blood Glucose; 0/Dietary Fats; 0/RNA, Messenger; 11061-68-0/Insulin; EC O-Palmitoyltransferase; EC Protein Kinases; EC Carboxylase

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