Document Detail


Aequorin-expressing yeast emits light under electric control.
MedLine Citation:
PMID:  21291926     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
In this study, we show the use of direct external electrical stimulation of a jellyfish luminescent calcium-activated protein, aequorin, expressed in a transgenic yeast strain. Yeast cultures were electrically stimulated through two electrodes coupled to a standard power generator. Even low (1.5V) electric pulses triggered a rapid light peak and serial light pulses were obtained after electric pulses were applied periodically, suggesting that the system is re-enacted after a short refraction time. These results open up a new scenario, in the very interphase between synthetic biology and cybernetics, in which complex cellular behavior might be subjected to electrical control.
Authors:
Cristina Vilanova; Angeles Hueso; Carles Palanca; Guillem Marco; Miguel Pitarch; Eduardo Otero; Juny Crespo; Jerzy Szablowski; Sara Rivera; Laura Domínguez-Escribà; Emilio Navarro; Arnau Montagud; Pedro Fernández de Córdoba; Asier González; Joaquín Ariño; Andrés Moya; Javier Urchueguía; Manuel Porcar
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-1-31
Journal Detail:
Title:  Journal of biotechnology     Volume:  -     ISSN:  1873-4863     ISO Abbreviation:  -     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-2-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8411927     Medline TA:  J Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier B.V.
Affiliation:
Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universitat de València, Postal Code 22085, 46071 València, Spain; Interdisciplinary Modeling Group, Intertech, Instituto Universitario de Matemática Pura y Aplicada, IUMPA, Departamento de Matemática Aplicada, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022 València, Spain.
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