Document Detail

Adverse effects of lupus anticoagulant positive blood sera on placental viability can be prevented by heparin in vitro.
MedLine Citation:
PMID:  15592302     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: Lupus anticoagulant poses a significant risk factor for obstetric complications, whereas heparin improves live birth rates in those pregnancies. Pathophysiology of antiphospholipid antibodies on placental function involves coagulopathies and thrombosis but also dysregulated trophoblast turnover. STUDY DESIGN: With the use of placental explant cultures, we assessed the effect of lupus anticoagulant positive sera (LA + sera) on apoptosis, mitosis, and invasion of trophoblast and determined the role of unfractionated heparin in regulating these functions. RESULTS: LA + sera were associated with increased placental apoptosis (TUNEL, M30 formation, DNA laddering). LA + sera decreased villous trophoblast proliferation and reduced extravillous trophoblast invasion through matrigel. Heparin attenuated LA + sera-induced apoptosis and facilitated trophoblast invasion. CONCLUSION: Lupus anticoagulant may impair placentation by increasing apoptosis, attenuating mitosis and reducing invasion of the trophoblast. The direct effects on trophoblast viability by heparin demonstrate an alternative biologic function for this anticoagulant and raise the possibility that anomalous trophoblast development may be therapeutically regulated.
Patrick Bose; Simon Black; Mamed Kadyrov; Clemens Bartz; Abdul Shlebak; Lesley Regan; Berthold Huppertz
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of obstetrics and gynecology     Volume:  191     ISSN:  0002-9378     ISO Abbreviation:  Am. J. Obstet. Gynecol.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-12-13     Completed Date:  2005-01-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370476     Medline TA:  Am J Obstet Gynecol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2125-31     Citation Subset:  AIM; IM    
Department of Anatomy, University Hospital, Aachen, Germany.
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MeSH Terms
Abortion, Spontaneous
Antibodies, Antiphospholipid / immunology*
Apoptosis / drug effects,  physiology*
Blotting, Western
DNA Fragmentation
Heparin / pharmacology*
In Situ Nick-End Labeling
Lupus Erythematosus, Systemic / blood*,  immunology
Middle Aged
Mitosis / physiology
Placenta / cytology,  drug effects
Pregnancy Trimester, First
Reference Values
Sensitivity and Specificity
Tissue Culture Techniques
Trophoblasts / drug effects*,  immunology
Reg. No./Substance:
0/Antibodies, Antiphospholipid; 9005-49-6/Heparin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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