| The adverse effect of phytoestrogens on the synthesis and secretion of ovarian oxytocin in cattle. | |
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MedLine Citation:
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PMID: 19799752 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The current investigations were undertaken to study the mechanism of the adverse effect of phytoestrogens on the function of bovine granulosa (follicles >1< cm in diameter) and luteal cells from day 1-5, 6-10, 11-15, 16-19 of the oestrous cycle. The cells were incubated with genistein, daidzein or coumestrol (each at the dose of 1 × 10(-6) m). The viability and secretion of estradiol (E2), progesterone (P4) and oxytocin (OT) were measured after 72 h of incubation. Moreover, the expression of mRNA for neurophysin-I/OT (NP-I/OT; precursor of OT) and peptidyl-glycine-α-amidating monooxygenase (PGA, an enzyme responsible for post-translational OT synthesis) was determined after 8 h of treatment. None of the phytoestrogens used affected the viability of cells except for coumestrol. The increased secretion of E2 and P4 was only obtained by coumestrol (p < 0.05) from granulosa cells from follicles <1 cm in diameter and decreased from luteal cells on days 11-15 of the oestrous cycle, respectively. All three phytoestrogens stimulated (p < 0.05) OT secretion from granulosa and luteal cells in all stages of the oestrous cycle and the expression of NP-I/OT mRNA in the both types of cells. The expression of mRNA for PGA was stimulated (p < 0.05) by daidzein and coumestrol in granulosa cells, and by genistein and coumestrol in luteal cells. In conclusion, our results demonstrate that these phytoestrogens can impair the ovary function in cattle by adversely affecting the synthesis of OT in follicles and in corpus luteum. However, their influence on the ovarian steroids secretion was less evident. |
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Authors:
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J Mlynarczuk; Mh Wrobel; J Kotwica |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Reproduction in domestic animals = Zuchthygiene Volume: 46 ISSN: 1439-0531 ISO Abbreviation: Reprod. Domest. Anim. Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-01-19 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9015668 Medline TA: Reprod Domest Anim Country: Germany |
Other Details:
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Languages: eng Pagination: 21-8 Citation Subset: IM |
Copyright Information:
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© 2009 Blackwell Verlag GmbH. |
Affiliation:
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Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Olsztyn, Poland. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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