| Advantages of a dual-tracer model over reference tissue models for binding potential measurement in tumors. | |
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MedLine Citation:
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PMID: 23022732 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The quantification of tumor molecular expression in vivo could have a significant impact for informing and monitoring emerging targeted therapies in oncology. Molecular imaging of targeted tracers can be used to quantify receptor expression in the form of a binding potential (BP) if the arterial input curve or a surrogate of it is also measured. However, the assumptions of the most common approaches (reference tissue models) may not be valid for use in tumors. In this study, the validity of reference tissue models is investigated for use in tumors experimentally and in simulations. Three different tumor lines were grown subcutaneously in athymic mice and the mice were injected with a mixture of an epidermal growth factor receptor-targeted fluorescent tracer and an untargeted fluorescent tracer. A one-compartment plasma input model demonstrated that the transport kinetics of both tracers was significantly different between tumors and all potential reference tissues, and using the reference tissue model resulted in a theoretical underestimation in BP of 50% ± 37%. On the other hand, the targeted and untargeted tracers demonstrated similar transport kinetics, allowing a dual-tracer approach to be employed to accurately estimate BP (with a theoretical error of 0.23% ± 9.07%). These findings highlight the potential for using a dual-tracer approach to quantify receptor expression in tumors with abnormal hemodynamics, possibly to inform the choice or progress of molecular cancer therapies. |
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Authors:
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K M Tichauer; K S Samkoe; W S Klubben; T Hasan; B W Pogue |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-10-01 |
Journal Detail:
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Title: Physics in medicine and biology Volume: 57 ISSN: 1361-6560 ISO Abbreviation: Phys Med Biol Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-10-03 Completed Date: 2013-02-21 Revised Date: 2013-05-30 |
Medline Journal Info:
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Nlm Unique ID: 0401220 Medline TA: Phys Med Biol Country: England |
Other Details:
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Languages: eng Pagination: 6647-59 Citation Subset: IM |
Affiliation:
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Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA. kenneth.tichauer@dartmouth.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Transport Cell Line, Tumor Mice Models, Biological* Molecular Imaging Neoplasms / diagnosis, metabolism*, pathology Protein Binding Radioactive Tracers Rats Reference Standards |
| Grant Support | |
ID/Acronym/Agency:
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P01 CA084203/CA/NCI NIH HHS; P01CA84201/CA/NCI NIH HHS; R01 CA156177/CA/NCI NIH HHS; R01CA156177/CA/NCI NIH HHS; U54 CA151662/CA/NCI NIH HHS; U54CA151662/CA/NCI NIH HHS; //Canadian Institutes of Health Research |
| Chemical | |
Reg. No./Substance:
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0/Radioactive Tracers |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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