Document Detail


Advantages of a dual-tracer model over reference tissue models for binding potential measurement in tumors.
MedLine Citation:
PMID:  23022732     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The quantification of tumor molecular expression in vivo could have a significant impact for informing and monitoring emerging targeted therapies in oncology. Molecular imaging of targeted tracers can be used to quantify receptor expression in the form of a binding potential (BP) if the arterial input curve or a surrogate of it is also measured. However, the assumptions of the most common approaches (reference tissue models) may not be valid for use in tumors. In this study, the validity of reference tissue models is investigated for use in tumors experimentally and in simulations. Three different tumor lines were grown subcutaneously in athymic mice and the mice were injected with a mixture of an epidermal growth factor receptor-targeted fluorescent tracer and an untargeted fluorescent tracer. A one-compartment plasma input model demonstrated that the transport kinetics of both tracers was significantly different between tumors and all potential reference tissues, and using the reference tissue model resulted in a theoretical underestimation in BP of 50% ± 37%. On the other hand, the targeted and untargeted tracers demonstrated similar transport kinetics, allowing a dual-tracer approach to be employed to accurately estimate BP (with a theoretical error of 0.23% ± 9.07%). These findings highlight the potential for using a dual-tracer approach to quantify receptor expression in tumors with abnormal hemodynamics, possibly to inform the choice or progress of molecular cancer therapies.
Authors:
K M Tichauer; K S Samkoe; W S Klubben; T Hasan; B W Pogue
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-10-01
Journal Detail:
Title:  Physics in medicine and biology     Volume:  57     ISSN:  1361-6560     ISO Abbreviation:  Phys Med Biol     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-03     Completed Date:  2013-02-21     Revised Date:  2013-10-23    
Medline Journal Info:
Nlm Unique ID:  0401220     Medline TA:  Phys Med Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  6647-59     Citation Subset:  IM    
Affiliation:
Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA. kenneth.tichauer@dartmouth.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Transport
Cell Line, Tumor
Mice
Models, Biological*
Molecular Imaging
Neoplasms / diagnosis,  metabolism*,  pathology
Protein Binding
Radioactive Tracers
Rats
Reference Standards
Grant Support
ID/Acronym/Agency:
P01 CA084203/CA/NCI NIH HHS; P01CA84201/CA/NCI NIH HHS; R01 CA109558/CA/NCI NIH HHS; R01 CA156177/CA/NCI NIH HHS; R01CA156177/CA/NCI NIH HHS; U54 CA105480/CA/NCI NIH HHS; U54 CA151662/CA/NCI NIH HHS; U54CA151662/CA/NCI NIH HHS; //Canadian Institutes of Health Research
Chemical
Reg. No./Substance:
0/Radioactive Tracers
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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