| Advantages of compound-specific stable isotope measurements over bulk measurements in studies on plant uptake of intact amino acids. | |
| | |
MedLine Citation:
|
PMID: 19757447 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Increasing interest in the ability of plants to take up amino acids has given rise to questions on the accuracy of the commonly used bulk method to measure and calculate amino acid uptake. This method uses bulk measurements of 13C and 15N enrichment in plant tissues after application of dual-labelled amino acids but some authors have recommended the use of compound-specific stable isotope (CSI) analysis of the plants' amino acids instead. However, there has never been a direct evaluation of both methods. We conducted a field study applying dual-labelled (13C, 15N) amino acids (glycine, valine, tyrosine and lysine) to soil of a Plantago lanceolata monoculture. Root and shoot samples were collected 24 h after label application and the isotope composition of the plant tissues was investigated using bulk and CSI measurements. Enrichment of 13C in the case of CSI measurements was limited to the applied amino acids, showing that no additional 13C had been incorporated into the plants' amino acid pool via the uptake of tracer-derived C-fragments. Compared with this rather conservative indicator of amino acid uptake, the 13C enrichment of bulk measurements was 8, 5, 1.6 and 6 times higher for fine roots, storage roots, shoot and the whole plant, respectively. These findings show that the additional uptake of tracer-derived C-fragments will result in a considerable overestimation of amino acid uptake in the case of bulk measurements. We therefore highly recommend the use of CSI measurements for future amino acid uptake studies due to their higher accuracy. |
| | |
Authors:
|
Leopold Sauheitl; Bruno Glaser; Alexandra Weigelt |
Related Documents
:
|
7824067 - Uptake of transmitter amino acids by glial plasmalemmal vesicles from different regions... 6621327 - Influence of exercise on chylomicron triacylglycerol metabolism: plasma turnover and mu... 1166067 - Aminoisobutyric acid transport in primary cultures of normal adult rat hepatocytes. 9025757 - Monitoring gene therapy with herpes simplex virus thymidine kinase in hepatoma cells: u... 679197 - Effects of retinoic acid and juvenile hormone on the induction of ornithine decarboxyla... 19254837 - Lemon juice improves the extractability and quality characteristics of pectin from yell... |
Publication Detail:
|
Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Rapid communications in mass spectrometry : RCM Volume: 23 ISSN: 1097-0231 ISO Abbreviation: Rapid Commun. Mass Spectrom. Publication Date: 2009 Oct |
Date Detail:
|
Created Date: 2009-09-23 Completed Date: 2009-11-25 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8802365 Medline TA: Rapid Commun Mass Spectrom Country: England |
Other Details:
|
Languages: eng Pagination: 3333-42 Citation Subset: IM |
Copyright Information:
|
Copyright 2009 John Wiley & Sons, Ltd. |
Affiliation:
|
University of Bayreuth, Soil Physics Section, Germany. leopold.sauheitl@uni-bayreuth.de |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acids
/
analysis,
metabolism* Carbon Isotopes / analysis*, metabolism Isotope Labeling / methods* Nitrogen Isotopes / analysis*, metabolism Plantago / chemistry, metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Amino Acids; 0/Carbon Isotopes; 0/Nitrogen Isotopes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Developments in asbestos cancer risk assessment.
Next Document: Functionalized pyrolytic highly oriented graphite polymer film for surface-assisted laser desorption...