Document Detail


Advancing mammalian cell culture engineering using genome-scale technologies.
MedLine Citation:
PMID:  17681628     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mammalian cell-derived protein therapeutic production has changed the landscape of human healthcare in the past two decades. The importance of protein therapeutics has motivated the search for more cost-effective and efficient cell lines capable of producing high quality protein products. The factors contributing to optimal producer cell lines are often complex, and not simply conferred by one gene or gene product, which makes an understanding of system-wide properties for better engineering of optimized cell lines essential. Genome-scale technologies (genomics, transcriptomics and proteomics) enable such engineering studies. However, the use of these technologies in cell culture engineering is still in its infancy. Here, we summarize current knowledge of cell properties important for the design of efficient protein-producing mammalian cell lines, and highlight relevant studies to-date that use genome-scale technologies in these cell systems. We also provide a focused review of relevant alternative and emerging technologies, which have seen limited use in cell culture engineering, but hold great potential for significant advancements in protein therapeutic production.
Authors:
Timothy J Griffin; Gargi Seth; Hongwei Xie; Sricharan Bandhakavi; Wei-Shou Hu
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review     Date:  2007-08-02
Journal Detail:
Title:  Trends in biotechnology     Volume:  25     ISSN:  0167-7799     ISO Abbreviation:  Trends Biotechnol.     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-08-27     Completed Date:  2007-10-30     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  8310903     Medline TA:  Trends Biotechnol     Country:  England    
Other Details:
Languages:  eng     Pagination:  401-8     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA. tgriffin@umn.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Humans
Oligonucleotide Array Sequence Analysis
Protein Engineering / methods*
Proteomics / methods
Recombinant Proteins / biosynthesis*,  therapeutic use
Grant Support
ID/Acronym/Agency:
AG25371/AG/NIA NIH HHS; DK073731/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Recombinant Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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