Document Detail


Advancing pluripotent stem cell culture: it is a matter of setting the standard.
MedLine Citation:
PMID:  23330735     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human pluripotent stem cells (hPSCs), defined by their ability to proliferate indefinitely and the capacity to differentiate into all tissue cell types of the adult, represent a platform for the realization of breakthrough technologies for industrial and regenerative medicine applications. We have witnessed tremendous developments over the last decade related to methods for establishment, maintenance, differentiation, and applications of hPSCs and their derivatives. Despite all progress made in the hPSC field, there are still fundamental issues yet to be resolved. For example, our understanding of the pluripotent state remains limited, which in turn may have substantial consequences on how we interpret and communicate scientific data concerning hPSCs. This brief commentary aims to highlight recent important findings that demonstrate additional levels of complexity to the current assessment of pluripotent stem cell cultures. In addition, these data may help to provide some explanations for the challenges in reproducing hPSC differentiation protocols across laboratories.
Authors:
Peter Sartipy
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-01-18
Journal Detail:
Title:  Stem cells and development     Volume:  22     ISSN:  1557-8534     ISO Abbreviation:  Stem Cells Dev.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-04-11     Completed Date:  2013-10-31     Revised Date:  2014-04-15    
Medline Journal Info:
Nlm Unique ID:  101197107     Medline TA:  Stem Cells Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1159-61     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Cell Culture Techniques / methods,  standards
Cell Differentiation / genetics*
Cell Lineage / genetics*
Gene Expression Profiling / methods*,  standards
Homeodomain Proteins / genetics
Humans
Octamer Transcription Factor-3 / genetics
Pluripotent Stem Cells / cytology,  metabolism*
Regenerative Medicine / methods,  standards
SOXB1 Transcription Factors / genetics
Chemical
Reg. No./Substance:
0/Homeodomain Proteins; 0/NANOG protein, human; 0/Octamer Transcription Factor-3; 0/POU5F1 protein, human; 0/SOX2 protein, human; 0/SOXB1 Transcription Factors
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