Document Detail


Advances on photosystem II investigation by measurement of delayed chlorophyll fluorescence by a phosphoroscopic methods.
MedLine Citation:
PMID:  12685657     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method for data acquisition based on recording of light signal from a conventional phophoroscope fluorometer with high-speed digitalization is proposed to extract more information from a delayed chlorophyll a fluorescence (DF) signal. During the signal processing from all points registered by the fluorometer, we obtain simultaneously a large number of induction curves of DF decaying at different time ranges. In addition, it is possible to register a series of dark relaxation kinetics of DF, recorded at different moments during the induction period or at different temperatures. This allows the evaluation of the contribution of DF kinetic components during the induction period or at different temperatures and the comparison between DF signals registered with different phophoroscopes. With the phosphoroscope system used in this study, we have shown that the contribution of the millisecond components (with lifetimes 0.6 and 2-4 ms) predominates during the first second of the induction period. After 1 s of illumination, the amplitudes of the 0.6 ms and 2-4 ms components and of the slower one (with lifetime more than 10 ms) become approximately equal. The change in lifetime of the different components during the induction and during gradual heating is also observed. It is shown that all registered DF kinetic components have different temperature dependences.
Authors:
Ivelina Zaharieva; Vassilij Goltsev
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Photochemistry and photobiology     Volume:  77     ISSN:  0031-8655     ISO Abbreviation:  Photochem. Photobiol.     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-04-10     Completed Date:  2003-05-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376425     Medline TA:  Photochem Photobiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  292-8     Citation Subset:  IM    
Affiliation:
Institute of Biophysics, Bulgarian Academy of Sciences, Sofia, Bulgaria.
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis
Chlorophyll / chemistry,  radiation effects
Fluorometry
Light-Harvesting Protein Complexes
Photochemistry
Photosynthetic Reaction Center Complex Proteins / chemistry,  radiation effects*
Photosystem II Protein Complex
Chemical
Reg. No./Substance:
0/Light-Harvesting Protein Complexes; 0/Photosynthetic Reaction Center Complex Proteins; 0/Photosystem II Protein Complex; 1406-65-1/Chlorophyll

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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