| Advances in the use of nucleic acid probes in diagnosis of viral diseases of man. Brief review. | |
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MedLine Citation:
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PMID: 3322234 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A variety of methods are now available for the preparation and labelling of viral nucleic acids for use as probes in diagnostic virology. Some of these are assessed including the use of synthetic oligonucleotides in place of molecularly cloned nucleic acids, and alternatives to labelling with radioactive isotopes such as biotin, enzymes and fluorochromes. Dot blot, sandwich, indirect sandwich and in situ hybridization are covered, and examples given of the current use of nucleic acid probes in detection of human viral infections. The potential and limitations of nucleic acid hybridization are discussed in the light of these new methods. |
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Authors:
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M Norval; R W Bingham |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Review |
Journal Detail:
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Title: Archives of virology Volume: 97 ISSN: 0304-8608 ISO Abbreviation: Arch. Virol. Publication Date: 1987 |
Date Detail:
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Created Date: 1988-02-09 Completed Date: 1988-02-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7506870 Medline TA: Arch Virol Country: AUSTRIA |
Other Details:
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Languages: eng Pagination: 151-65 Citation Subset: IM |
Affiliation:
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Department of Bacteriology, University of Edinburgh Medical School. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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DNA, Viral
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analysis*,
genetics Humans RNA, Viral / analysis*, genetics Virus Diseases / diagnosis* Viruses / genetics |
| Chemical | |
Reg. No./Substance:
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0/DNA, Viral; 0/RNA, Viral |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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