Document Detail

Advances in oocyte cryopreservation--part II: rapid cooling using vitrification.
MedLine Citation:
PMID:  19405208     Owner:  NLM     Status:  MEDLINE    
PURPOSE: The need for freezing oocytes has been established for females undergoing potential therapy that could damage their ovarian egg reserve, for ethical or religious reasons (not having excess embryos frozen) or for women nearing the age of lower fecundity but not married and not ready to use donor sperm. Applying cryopreservation techniques for oocytes used for embryos resulted in very poor pregnancy results. A rapid flash freezing technique has rekindled interest in oocyte freezing known as vitrification. METHODS: Certain modifications, especially minimizing the volume, have resulted in marked improved pregnancy rates with vitrified thawed oocytes. The lower volume allows decreased exposure to the toxic cryoprotection. Commercial interests have developed an effective device called cryotop but some concerns about microorganism contamination exist because it is an open system. Modifications have been made to make available the cryotip, a closed device which addresses the contamination issue. RESULTS: Frozen oocyte survival rates upon thawing fertilization rates and subsequent pregnancy rates after embryo transfer have been reported comparable to data with frozen thawed embryos. CONCLUSIONS: Because of the uncertainty of the programmable freezer used for the slow cool method and because there has been more commercial interest in the vitrification method, the "flash" freeze protocol seems to have an edge over the slow cool method for oocyte freezing.
J H Check
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Publication Detail:
Type:  Editorial    
Journal Detail:
Title:  Clinical and experimental obstetrics & gynecology     Volume:  36     ISSN:  0390-6663     ISO Abbreviation:  Clin Exp Obstet Gynecol     Publication Date:  2009  
Date Detail:
Created Date:  2009-04-29     Completed Date:  2009-06-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7802110     Medline TA:  Clin Exp Obstet Gynecol     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  5-9     Citation Subset:  IM    
The University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School at Camden, Cooper Hospital/University Medical Center, Dept. Obstet & Gynecol, Division of Reproductive Endocrinology & Infertility, Camden, NJ, USA.
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MeSH Terms
Cryopreservation / methods*
Fertilization in Vitro

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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