Document Detail


Advances in microscopy: microautoradiography of single cells.
MedLine Citation:
PMID:  16260295     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Microautoradiography (MAR) is an efficient method to obtain reliable information about the ecophysiology of microorganisms at the single cell level in mixed communities. Data obtained by the traditional MAR method can now be improved significantly when MAR is combined with fluorescence in situ hybridization (FISH) with oligonucleotide probes for the identification of target organisms. This chapter discusses how to use MAR-FISH in various ecosystems with emphasis on the type of information to be obtained, incubation conditions, and detailed protocols for the MAR technique. It also discusses new MAR applications and the type of information that can be obtained, e.g., the use of dual labeling to investigate the simultaneous uptake of several substrates by individual prokaryotes.
Authors:
Jeppe Lund Nielsen; Per Halkjaer Nielsen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in enzymology     Volume:  397     ISSN:  0076-6879     ISO Abbreviation:  Meth. Enzymol.     Publication Date:  2005  
Date Detail:
Created Date:  2005-11-01     Completed Date:  2005-12-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0212271     Medline TA:  Methods Enzymol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  237-56     Citation Subset:  IM    
Affiliation:
Department of Life Sciences, Aalborg University, Denmark.
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MeSH Terms
Descriptor/Qualifier:
Acetic Acid / metabolism
Autoradiography / methods*
Biodegradation, Environmental
Biomass
Carbon Dioxide / metabolism
Carbon Radioisotopes
Ecosystem
In Situ Hybridization, Fluorescence
Isotope Labeling
Microscopy / methods
Microscopy, Confocal / methods
Propionates / metabolism
Sewage / microbiology
Tritium
Chemical
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Propionates; 0/Sewage; 10028-17-8/Tritium; 124-38-9/Carbon Dioxide; 64-19-7/Acetic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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