| Advances in microscopy: microautoradiography of single cells. | |
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MedLine Citation:
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PMID: 16260295 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Microautoradiography (MAR) is an efficient method to obtain reliable information about the ecophysiology of microorganisms at the single cell level in mixed communities. Data obtained by the traditional MAR method can now be improved significantly when MAR is combined with fluorescence in situ hybridization (FISH) with oligonucleotide probes for the identification of target organisms. This chapter discusses how to use MAR-FISH in various ecosystems with emphasis on the type of information to be obtained, incubation conditions, and detailed protocols for the MAR technique. It also discusses new MAR applications and the type of information that can be obtained, e.g., the use of dual labeling to investigate the simultaneous uptake of several substrates by individual prokaryotes. |
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Authors:
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Jeppe Lund Nielsen; Per Halkjaer Nielsen |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in enzymology Volume: 397 ISSN: 0076-6879 ISO Abbreviation: Meth. Enzymol. Publication Date: 2005 |
Date Detail:
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Created Date: 2005-11-01 Completed Date: 2005-12-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0212271 Medline TA: Methods Enzymol Country: United States |
Other Details:
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Languages: eng Pagination: 237-56 Citation Subset: IM |
Affiliation:
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Department of Life Sciences, Aalborg University, Denmark. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetic Acid
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metabolism Autoradiography / methods* Biodegradation, Environmental Biomass Carbon Dioxide / metabolism Carbon Radioisotopes Ecosystem In Situ Hybridization, Fluorescence Isotope Labeling Microscopy / methods Microscopy, Confocal / methods Propionates / metabolism Sewage / microbiology Tritium |
| Chemical | |
Reg. No./Substance:
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0/Carbon Radioisotopes; 0/Propionates; 0/Sewage; 10028-17-8/Tritium; 124-38-9/Carbon Dioxide; 64-19-7/Acetic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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