Document Detail


Adult peripheral blood mononuclear cells transdifferentiate in vitro and integrate into the retina in vivo.
MedLine Citation:
PMID:  21247410     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Adult peripheral blood-derived cells are able to differentiate into a variety of cell types, including nerve cells, liver-like cells and epithelial cells. However, their differentiation into retina-like cells is controversial. In the present study, transdifferentiation potential of human adult peripheral blood mononuclear cells into retina-like cells and integration into the retina of mice were investigated. Freshly isolated adult peripheral blood mononuclear cells were divided into two groups: cells in group I were cultured in neural stem cell medium, and cells in group II were exposed to conditioned medium from rat retinal tissue culture. After 5 days, several distinct cell morphologies were observed, including standard mononuclear, neurons with one or two axons and elongated glial-like cells. Immunohistochemical analysis of neural stem cell, neuron and retina cell markers demonstrated that cells in both groups were nestin-, MAP2 (microtubule-associated protein)- and GFAP (glial fibrillary acidic protein)-positive. Flow cytometry results suggested a significant increase in nestin-, MAP2- and CD16-positive cells in group I and nestin-, GFAP-, MAP2-, vimentin- and rhodopsin-positive cells in group II. To determine survival, migration and integration in vivo, cell suspensions (containing group I or group II cells) were injected into the vitreous or the peritoneum. Tissue specimens were obtained and immunostained 4 weeks after transplantation. We found that cells delivered by intravitreal injection integrated into the retina. Labelled cells were not detected in the retina of mice receiving differentiated cells by intraperitoneal injection, but cells (groups I and II) were detected in the liver and spleen. Our findings revealed that human adult peripheral blood mononuclear cells could be induced to transdifferentiate into neural precursor cells and retinal progenitor cells in vitro, and the differentiated peripheral blood mononuclear cells can migrate and integrate into the retina in vivo.
Authors:
Qian Liu; Liping Guan; Bing Huang; Weihua Li; Qiao Su; Minbin Yu; Xiaoping Xu; Ting Luo; Shaochun Lin; Xuerong Sun; Mengfei Chen; Xigu Chen
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biology international     Volume:  35     ISSN:  1095-8355     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-05-12     Completed Date:  2011-09-02     Revised Date:  2012-08-02    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  631-8     Citation Subset:  IM    
Affiliation:
State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yatsen University, Guangzhou 510060, Peoples Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Markers / metabolism
Cell Transdifferentiation*
Cells, Cultured
Glial Fibrillary Acidic Protein / metabolism
Humans
Intermediate Filament Proteins / metabolism
Leukocytes, Mononuclear / cytology*
Mice
Microtubule-Associated Proteins / metabolism
Nerve Tissue Proteins / metabolism
Neurons / pathology
Rats
Retina / cytology*,  metabolism
Rhodopsin / metabolism
Vimentin / metabolism
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Glial Fibrillary Acidic Protein; 0/Intermediate Filament Proteins; 0/MAP2 protein, human; 0/Microtubule-Associated Proteins; 0/Nerve Tissue Proteins; 0/Vimentin; 0/nestin; 9009-81-8/Rhodopsin

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