Document Detail


Adult mouse epicardium modulates myocardial injury by secreting paracrine factors.
MedLine Citation:
PMID:  21505261     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The epicardium makes essential cellular and paracrine contributions to the growth of the fetal myocardium and the formation of the coronary vasculature. However, whether the epicardium has similar roles postnatally in the normal and injured heart remains enigmatic. Here, we have investigated this question using genetic fate-mapping approaches in mice. In uninjured postnatal heart, epicardial cells were quiescent. Myocardial infarction increased epicardial cell proliferation and stimulated formation of epicardium-derived cells (EPDCs), which remained in a thickened layer on the surface of the heart. EPDCs did not adopt cardiomyocyte or coronary EC fates, but rather differentiated into mesenchymal cells expressing fibroblast and smooth muscle cell markers. In vitro and in vivo assays demonstrated that EPDCs secreted paracrine factors that strongly promoted angiogenesis. In a myocardial infarction model, EPDC-conditioned medium reduced infarct size and improved heart function. Our findings indicate that epicardium modulates the cardiac injury response by conditioning the subepicardial environment, potentially offering a new therapeutic strategy for cardiac protection.
Authors:
Bin Zhou; Leah B Honor; Huamei He; Qing Ma; Jin-Hee Oh; Catherine Butterfield; Ruei-Zeng Lin; Juan M Melero-Martin; Elena Dolmatova; Heather S Duffy; Alexander von Gise; Pingzhu Zhou; Yong Wu Hu; Gang Wang; Bing Zhang; Lianchun Wang; Jennifer L Hall; Marsha A Moses; Francis X McGowan; William T Pu
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-04-18
Journal Detail:
Title:  The Journal of clinical investigation     Volume:  121     ISSN:  1558-8238     ISO Abbreviation:  J. Clin. Invest.     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-05-03     Completed Date:  2011-07-12     Revised Date:  2014-04-08    
Medline Journal Info:
Nlm Unique ID:  7802877     Medline TA:  J Clin Invest     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1894-904     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Fibroblasts / metabolism
Heart / physiology
Heart Injuries / metabolism
Homeostasis
Intercellular Signaling Peptides and Proteins / metabolism
Mice
Models, Biological
Models, Genetic
Myocardial Infarction / pathology
Myocardium / metabolism,  pathology*
Myocytes, Cardiac / metabolism*
Neovascularization, Pathologic
Pericardium / metabolism*
Treatment Outcome
Grant Support
ID/Acronym/Agency:
P01 CA045548/CA/NCI NIH HHS; P41 RR005351/RR/NCRR NIH HHS; R01 HL093339/HL/NHLBI NIH HHS; R01 HL094683/HL/NHLBI NIH HHS; R01 HL094683/HL/NHLBI NIH HHS; U01 HL100401/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Intercellular Signaling Peptides and Proteins
Comments/Corrections

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