| Adult human pancreatic duct and islet cells exhibit similarities in expression and differences in phosphorylation and complex formation of the homeodomain protein Ipf-1. | |
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MedLine Citation:
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PMID: 10871194 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The homeodomain transcription factor encoded by the pancreatic and duodenal homeobox gene-1 (Ipf-1) is essential for pancreatic ontogenesis. Whether Ipf-1 is also involved in the neogenesis of beta-cells in the adult pancreas is unknown. We examined whether Ipf-1 is expressed in adult human pancreatic ducts, which are thought to generate new beta-cells. In tissue sections, virtually all duct cells were immunopositive for Ipf-1, as were the islet beta-cells but not the acinar cells. After isolation and culture, both duct and islet cell preparations contained the Ipf-1 immunoreactive proteins p42 and p45 (42 and 45 kDa, respectively) in similar proportions, but the expression levels were twofold lower in duct cells. After 4 h of labeling, the endocrine cells exhibited a sevenfold higher phosphorylation of p42 than the duct cells, whereas p45 was phosphorylated only in endocrine cells. Homeobox binding transcription factor complexes with Ipf-1 in duct cells differed from those in endocrine cells in terms of gel mobility, sequence specificity, and affinity. The observed similarities in Ipf-1 expression by adult human pancreatic duct cells and endocrine cells may reflect their common ontogenic origin, whereas the differences in Ipf-1 phosphorylation and complex formation may correlate with their divergent differentiation. |
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Authors:
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H Heimberg; L Bouwens; Y Heremans; M Van De Casteele; V Lefebvre; D Pipeleers |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Diabetes Volume: 49 ISSN: 0012-1797 ISO Abbreviation: Diabetes Publication Date: 2000 Apr |
Date Detail:
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Created Date: 2000-07-06 Completed Date: 2000-07-06 Revised Date: 2011-06-01 |
Medline Journal Info:
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Nlm Unique ID: 0372763 Medline TA: Diabetes Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 571-9 Citation Subset: AIM; IM |
Affiliation:
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Diabetes Research Center, Vrije Universiteit Brussel, Belgium. hheimber@vub.vub.ac.be |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Child Gene Expression* Homeodomain Proteins* / genetics, metabolism Humans Islets of Langerhans / metabolism* Middle Aged Pancreas / metabolism Pancreatic Ducts / metabolism* Phosphorylation Trans-Activators / genetics*, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Homeodomain Proteins; 0/Trans-Activators; 0/pancreatic and duodenal homeobox 1 protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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