Document Detail


Adult human pancreatic duct and islet cells exhibit similarities in expression and differences in phosphorylation and complex formation of the homeodomain protein Ipf-1.
MedLine Citation:
PMID:  10871194     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The homeodomain transcription factor encoded by the pancreatic and duodenal homeobox gene-1 (Ipf-1) is essential for pancreatic ontogenesis. Whether Ipf-1 is also involved in the neogenesis of beta-cells in the adult pancreas is unknown. We examined whether Ipf-1 is expressed in adult human pancreatic ducts, which are thought to generate new beta-cells. In tissue sections, virtually all duct cells were immunopositive for Ipf-1, as were the islet beta-cells but not the acinar cells. After isolation and culture, both duct and islet cell preparations contained the Ipf-1 immunoreactive proteins p42 and p45 (42 and 45 kDa, respectively) in similar proportions, but the expression levels were twofold lower in duct cells. After 4 h of labeling, the endocrine cells exhibited a sevenfold higher phosphorylation of p42 than the duct cells, whereas p45 was phosphorylated only in endocrine cells. Homeobox binding transcription factor complexes with Ipf-1 in duct cells differed from those in endocrine cells in terms of gel mobility, sequence specificity, and affinity. The observed similarities in Ipf-1 expression by adult human pancreatic duct cells and endocrine cells may reflect their common ontogenic origin, whereas the differences in Ipf-1 phosphorylation and complex formation may correlate with their divergent differentiation.
Authors:
H Heimberg; L Bouwens; Y Heremans; M Van De Casteele; V Lefebvre; D Pipeleers
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Diabetes     Volume:  49     ISSN:  0012-1797     ISO Abbreviation:  Diabetes     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2000-07-06     Completed Date:  2000-07-06     Revised Date:  2011-06-01    
Medline Journal Info:
Nlm Unique ID:  0372763     Medline TA:  Diabetes     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  571-9     Citation Subset:  AIM; IM    
Affiliation:
Diabetes Research Center, Vrije Universiteit Brussel, Belgium. hheimber@vub.vub.ac.be
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Child
Gene Expression*
Homeodomain Proteins* / genetics,  metabolism
Humans
Islets of Langerhans / metabolism*
Middle Aged
Pancreas / metabolism
Pancreatic Ducts / metabolism*
Phosphorylation
Trans-Activators / genetics*,  metabolism*
Chemical
Reg. No./Substance:
0/Homeodomain Proteins; 0/Trans-Activators; 0/pancreatic and duodenal homeobox 1 protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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