| Adult bone marrow stem/progenitor cells (MSCs) are preconditioned by microenvironmental "niches" in culture: a two-stage hypothesis for regulation of MSC fate. | |
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MedLine Citation:
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PMID: 16046665 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mesenchymal stem cells (MSCs) are clonal, plastic adherent cells from bone marrow that can differentiate into various tissue lineages, including osteoblasts, adipocytes, chondrocytes, myoblasts, hepatocytes, and possibly even neural cells. Because MSCs are multipotent and their numbers are easily expanded in culture, there has been much interest in their clinical potential for tissue repair and gene therapy. Consequently, numerous studies have been carried out demonstrating the migration and multiorgan engraftment potential of MSCs in animal models and in human clinical trials. Understanding the mechanisms behind MSC cell fate determination is not easy, because the molecular processes that drive engraftment and differentiation are complex. Even in an in vitro system, the molecular cues necessary to induce differentiation are not easily identified or reproduced. In this Perspective, we emphasize the importance of microenvironmental factors in culture and suggest that MSC differentiation in vitro is regulated by a two-stage mechanism involving preconditioning by factors in the culture microenvironment followed by response to soluble differentiating factors. |
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Authors:
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Carl A Gregory; Joni Ylostalo; Darwin J Prockop |
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Publication Detail:
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Type: Journal Article; Review Date: 2005-07-26 |
Journal Detail:
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Title: Science's STKE : signal transduction knowledge environment Volume: 2005 ISSN: 1525-8882 ISO Abbreviation: Sci. STKE Publication Date: 2005 Jul |
Date Detail:
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Created Date: 2005-07-27 Completed Date: 2006-03-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100964423 Medline TA: Sci STKE Country: United States |
Other Details:
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Languages: eng Pagination: pe37 Citation Subset: IM |
Affiliation:
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Center for Gene Therapy, Tulane University Health Sciences Center, 1430 Tulane Avenue, New Orleans, LA 70112, USA. cgregory@tulane.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Animals Bone Marrow Cells / cytology*, drug effects Cell Adhesion Cell Count Cell Differentiation / drug effects, physiology Cell Lineage / drug effects, physiology* Cells, Cultured / cytology, drug effects Culture Media / pharmacology Gene Expression Profiling Humans Intercellular Signaling Peptides and Proteins / physiology Mesenchymal Stem Cell Transplantation Mesenchymal Stem Cells / cytology*, drug effects Models, Biological* Organ Specificity |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/DKK1 protein, human; 0/Intercellular Signaling Peptides and Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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