| Adsorptive depletion of alpha4 integrin(hi)- and CX3CR1hi-expressing proinflammatory monocytes in patients with ulcerative colitis. | |
| | |
MedLine Citation:
|
PMID: 19908144 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND: Two main functionally distinct monocytes phenotypes are known: the CD14(hi)CD16(-) "classical" and the CD14(+)CD16(+) "proinflammatory" phenotypes. The latter phenotype is elevated in patients with ulcerative colitis (UC) and is suspected to have a major role in the immunopathogenesis of UC. AIM: To selectively deplete circulating proinflammatory CD14(+)CD16(+) monocyte phenotype. METHODS: Seven corticosteroid-naïve patients with UC (clinical activity index = 8.7 +/- 1.3) and seven healthy subjects were included. In patients with UC, granulocyte/monocyte adsorption (GMA) was done with an Adacolumn that selectively adsorbs leucocytes of the myeloid lineage. Blood from all subjects at baseline and from the patients immediately after the first GMA session was processed. Isolated monocytes were subjected to fluorescence-activated cell sorter analyses. RESULTS: The seven UC patients achieved remission (CAI <or=4) after 5-10 GMA sessions. GMA induced a strong fall in the ratio (%) of CD14(+)CD16(+) to CD14(hi)CD16(-) monocytes, from 10.0 +/- 1.4 to 3.0 +/- 0.9. Further, expressions of alpha4 integrin (374.8 +/- 26.1 mean fluorescence intensity, MFI) and CX(3)CR1 (49.5 +/- 4.6 MFI) were significantly high on CD14(+)CD16(+)monocytes as compared with on CD14(hi)CD16(-) monocytes (169.2 +/- 17.2 and 33.2 +/- 3.6 MFI, respectively). Additionally, GMA significantly increased the ratio of the CD14(hi)CD16(-)CCR2(low) "immature" monocytes from 3.74 +/- 0.62 to 8.11 +/- 0.56 MFI. CONCLUSIONS: We found high expressions of alpha4 integrin and CX(3)CR1 on monocytes in patients with active UC, known to promote the extravasation of CD14(+)CD16(+) monocytes into the mucosa. GMA effectively depletes CD14(+)CD16(+) monocytes and concomitantly increases CD14(hi)CD16(-)CCR2(low) "immature" monocytes; thus GMA was associated with the emergence of less inflammatory monocyte phenotype in circulation. |
| | |
Authors:
|
Shin-ichiro Takeda; Toru Sato; Tatsuro Katsuno; Tomoo Nakagawa; Yoshiko Noguchi; Osamu Yokosuka; Yasushi Saito |
Related Documents
:
|
19853354 - Evaluation of phagocytes in atopic dermatitis. 12695164 - Primary sjögren's syndrome associated agranulocytosis: a benign disorder? 6463914 - Alveolar macrophage dysfunction in malignant lung tumours. 7144064 - Critical analysis of granulocyte function in 154 patients wtih different diseases. 2916434 - Two-dimensional echocardiographic findings in systemic sarcoidosis. 1127994 - Carotid endarterectomy. complications and preoperative assessment of risk. |
Publication Detail:
|
Type: Comparative Study; Journal Article Date: 2009-11-12 |
Journal Detail:
|
Title: Digestive diseases and sciences Volume: 55 ISSN: 1573-2568 ISO Abbreviation: Dig. Dis. Sci. Publication Date: 2010 Jul |
Date Detail:
|
Created Date: 2010-06-17 Completed Date: 2010-07-21 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7902782 Medline TA: Dig Dis Sci Country: United States |
Other Details:
|
Languages: eng Pagination: 1886-95 Citation Subset: AIM; IM |
Affiliation:
|
Department of Clinical Cell Biology, Graduate School of Medicine, Chiba University, Chuo-ku, Chiba-shi, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adolescent Adsorption Adult Antigens, CD14 / immunology*, metabolism Case-Control Studies Colitis, Ulcerative / blood*, physiopathology, therapy* Female Flow Cytometry Granulocytes / immunology, metabolism Humans Immunosuppressive Agents / therapeutic use Integrin alpha4 / immunology*, metabolism Leukapheresis / methods* Male Middle Aged Monocytes / immunology, metabolism Probability Receptors, CXCR3 / immunology*, metabolism Receptors, IgG / immunology, metabolism Statistics, Nonparametric Young Adult |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, CD14; 0/CXCR3 protein, human; 0/Immunosuppressive Agents; 0/Receptors, CXCR3; 0/Receptors, IgG; 143198-26-9/Integrin alpha4 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Women's decisions regarding tamoxifen for breast cancer prevention: responses to a tailored decision...
Next Document: Microstructure and In Vitro Beta Carotene Bioaccessibility of Heat Processed Orange Fleshed Sweet Po...