Document Detail

Adsorption of small biological molecules on silica from diluted aqueous solutions: quantitative characterization and implications to the Bernal's hypothesis.
MedLine Citation:
PMID:  7659402     Owner:  NLM     Status:  MEDLINE    
To describe quantitatively the adsorption of prebiotically important compounds of low molecular weight (amino acids, short linear peptides, cyclic dipeptides, the Krebs's cycle and other carboxylic acids, nucleosides and related phosphates) on silica surface from diluted neutral aqueous solutions, equilibrium constants (K) and free energies (-delta G) of adsorption were determined from the retention values measured by means of high-performance liquid chromatography on a silica gel column and from the isotherms measured under static conditions. For most carboxylic acids (including amino acids and linear peptides) -delta G values were negative and K < 1, thus showing very weak adsorption. Cyclic dipeptides (2,5-piperazinediones) exhibited higher adsorbability; -delta G > 0 and K > 1 were found for most of them. Influence of the structure of alpha-substituent on the adsorbability is analyzed. A linear dependence of -delta G on the number of aliphatic carbon atoms in a sorbate molecule was found for the series of aliphatic bifunctional amino acids, related dipeptides and 2,5-piperazinediones, as well as for the row from glycine to triglycyl glycine. The adsorption of nucleosides and their phosphates is characterized by much higher K and -delta G values (of the order of 10(2) and 10(4), respectively). The adsorption data available from our work and literature are summarized and discussed with implications to the Bernal's hypothesis on the roles of solid surfaces in the prebiotic formation of biopolymers from monomeric 'building blocks'.
V A Basiuk; Gromovoy TYu; E G Khil'chevskaya
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life     Volume:  25     ISSN:  0169-6149     ISO Abbreviation:  Orig Life Evol Biosph     Publication Date:  1995 Aug 
Date Detail:
Created Date:  1995-10-02     Completed Date:  1995-10-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8610391     Medline TA:  Orig Life Evol Biosph     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  375-93     Citation Subset:  IM; S    
Instituto de Ciencias Nucleares, Universidad Nacional Autonoma de Mexico, Mexico, D.F.
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MeSH Terms
Amino Acid Sequence
Amino Acids / isolation & purification
Biopolymers / chemistry,  isolation & purification*
Carboxylic Acids / isolation & purification
Models, Biological
Molecular Sequence Data
Nucleosides / isolation & purification
Oligopeptides / chemistry,  isolation & purification
Silicon Dioxide
Reg. No./Substance:
0/Amino Acids; 0/Biopolymers; 0/Carboxylic Acids; 0/Nucleosides; 0/Oligopeptides; 0/Solutions; 7631-86-9/Silicon Dioxide; 7732-18-5/Water

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