Document Detail

Adsorption of proteins at the oil/water interface--observation of protein adsorption by interfacial shear stress measurements.
MedLine Citation:
PMID:  20434317     Owner:  NLM     Status:  MEDLINE    
The interfacial adsorption of proteins is a problem during processing and formulation. The flexibility and aggregation of the protein cause the formation of a viscoelastic multilayer upon adsorption to the oil/water interface. Protein adsorption is a complex process and therefore it is difficult to elaborate which protein characteristics are important for the interfacial protein adsorption. From our results it seems that the molecular weight influences this the most. In this study, the adsorption of three proteins, bovine serum albumin (BSA), lysozyme and insulin, to oil/water interfaces is characterized by interfacial shear stress measurements using a sensitive rheometer equipped with a Du Noüy ring geometry. The interfacial protein adsorption is concentration dependent for the three proteins investigated, where the complex viscosity of the viscoelastic multilayer increases with increased concentration. The adsorption rate of the proteins varies, BSA reaches a plateau after 2h, insulin takes 24h to reach a plateau value and lysozyme adsorbs fast in the beginning, then levels of to a slow but steady increase in the complex viscosity, BSA and insulin were shown to have similar characteristics in the evolvement of the interfacial layer despite the rate, indicating similar mechanism of adsorption, whereas lysozyme differs. This is also seen when G' and G'' are used in order to relate the rheological measurements to the three adsorption regimes. This study shows the potentials and effectiveness of the Du Noüy ring geometry for characterization of protein adsorption to oil/water interfaces.
Stefania G Baldursdottir; Maria Stoier Fullerton; Signe Hougaard Nielsen; Lene Jorgensen
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Publication Detail:
Type:  Journal Article     Date:  2010-04-14
Journal Detail:
Title:  Colloids and surfaces. B, Biointerfaces     Volume:  79     ISSN:  1873-4367     ISO Abbreviation:  Colloids Surf B Biointerfaces     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-05-24     Completed Date:  2010-08-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9315133     Medline TA:  Colloids Surf B Biointerfaces     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  41-6     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier B.V. All rights reserved.
Department of Pharmaceutics and Analytical Chemistry, Faculty of Pharmaceutical Sciences, University of Copenhagen, Copenhagen, Denmark.
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MeSH Terms
Insulin / chemistry
Models, Chemical
Muramidase / chemistry
Oils / chemistry*
Proteins / chemistry*,  pharmacokinetics
Serum Albumin, Bovine
Stress, Mechanical
Surface Properties
Time Factors
Water / chemistry*
Reg. No./Substance:
0/Oils; 0/Proteins; 0/Serum Albumin, Bovine; 11061-68-0/Insulin; 7732-18-5/Water; EC

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