| Adsorption of Zearalenone by beta-D-glucans in the Saccharomyces cerevisiae cell wall. | |
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MedLine Citation:
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PMID: 15222549 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell walls of yeasts and bacteria are able to complex with mycotoxins and limit their bioavailability in the digestive tract when these yeasts and bacteria are given as feed additives to animals. To identify the component(s) of the yeast cell wall and the chemical interaction(s) involved in complex formation with zearalenone, four strains of Saccharomyces cerevisiae differing in their cell wall glucan and mannan content were tested. Laboratory strains wt292, fks1, and mnn9 were compared with industrial S. cerevisiae strain sc1026. The complex-forming capacity of the yeast cell walls was determined in vitro by modelling the plots of amount of toxin bound versus amount of toxin added using Hill's model. A cooperative relationship between toxin and adsorbent was shown, and a correlation between the amount of beta-D-glucans in cell walls and complex-forming efficacy was revealed (R2 = 0.889). Cell walls of strains wt292 and mnn9, which have higher levels of beta-D-glucans, were able to complex larger amounts of zearalenone, with higher association constants and higher affinity rates than those of the fks1 and sc1026 strains. The high chitin content in strains mnn9 and fks1 increased the alkali insolubility of beta-D-glucans from isolated cell walls and decreased the flexibility of these cell walls, which restricted access of zearalenone to the chemical sites of the beta-D-glucans involved in complex formation. The strains with high chitin content thus had a lower complex-forming capacity than expected based on their beta-D-glucans content. Cooperativity and the three-dimensional structure of beta-D-glucans indicate that weak noncovalent bonds are involved in the complex-forming mechanisms associated with zearalenone. The chemical interactions between beta-D-glucans and zearalenone are therefore more of an adsorption type than a binding type. |
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Authors:
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A Yiannikouris; J François; L Poughon; C G Dussap; G Bertin; G Jeminet; J P Jouany |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of food protection Volume: 67 ISSN: 0362-028X ISO Abbreviation: J. Food Prot. Publication Date: 2004 Jun |
Date Detail:
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Created Date: 2004-06-29 Completed Date: 2004-09-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7703944 Medline TA: J Food Prot Country: United States |
Other Details:
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Languages: eng Pagination: 1195-200 Citation Subset: IM |
Affiliation:
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UR1053 INRA, Unité de Recherches sur les Herbivores, Centre de Clermont-Theix, 63122 St. Genès Champanelle, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adsorption Animal Feed Animals Biological Availability Cell Wall / chemistry* Consumer Product Safety Food Microbiology Glucans / chemistry* Humans Saccharomyces cerevisiae / chemistry* Solubility Zearalenone / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Glucans; 17924-92-4/Zearalenone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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