Document Detail

Adrenocorticotropin and adenosine 3',5'-monophosphate stimulate de novo synthesis of adrenal phosphatidic acid by a cycloheximide-sensitive, CA++-dependent mechanism.
MedLine Citation:
PMID:  6273120     Owner:  NLM     Status:  MEDLINE    
We tested further our postulate that enhanced de novo synthesis of phosphatidic acid is responsible for ACTH- and cAMP-induced increases in adrenal phospholipids in the phosphatidate polyphosphoinositide pathway. During incubation of adrenal sections or cells in vitro, ACTH and cAMP increased the concentrations of and incorporation of [3H]glycerol and [14C]palmitate into phosphatidylcholine and phosphatidylethanolamine, two major phospholipids which are derived from phosphatidic acid, but are extrinsic to the inositide pathway. Thus, it is unlikely that ACTH and cAMP increase inositide phospholipids at the expense of other phospholipids. Similar to previously reported effects on phosphatidic acid and inositide phospholipids, cycloheximide blocked the effects of ACTH and cAMP on phosphatidylcholine and phosphatidylethanolamine. In addition, Ca++ was required for these effects, as well as for cAMP-induced increases in phosphatidic acid, inositide phospholipids, and steroidogenesis. Our findings strongly suggest that ACTH, via cAMP, stimulates de novo phosphatidate synthesis by a cycloheximide-sensitive, Ca++-dependent process, and this stimulation causes a rapid generalized increase in adrenal phospholipids. Moreover, the increased incorporation of labeled glycerol and palmitate into phospholipids suggests that ACTH and cAMP may stimulate the glycerol-3'-PO4 acyltransferase reaction. This stimulatory effect may play a central role in the steroidogenic and trophic actions of ACTH and cAMP.
R V Farese; M A Sabir; R E Larson
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Endocrinology     Volume:  109     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1981 Dec 
Date Detail:
Created Date:  1982-02-22     Completed Date:  1982-02-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1895-901     Citation Subset:  AIM; IM    
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MeSH Terms
Adrenal Glands / drug effects,  metabolism*
Adrenocorticotropic Hormone / pharmacology*
Calcium / pharmacology*
Corticosterone / biosynthesis
Cyclic AMP / pharmacology*
Cycloheximide / pharmacology*
Glycerol / metabolism
Phosphatidic Acids / biosynthesis*
Phospholipids / biosynthesis
Rats, Inbred Strains
Reg. No./Substance:
0/Phosphatidic Acids; 0/Phospholipids; 10028-17-8/Tritium; 50-22-6/Corticosterone; 56-81-5/Glycerol; 60-92-4/Cyclic AMP; 66-81-9/Cycloheximide; 7440-70-2/Calcium; 9002-60-2/Adrenocorticotropic Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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